Detailed information of TA system TA215252

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-timR/SymE(toxin)
• Location: 4304979..4305391
• Replicon: chromosome
• Accession: NZ_CP082824
• Organism: Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: K8B79_RS21060
• Protein ID: WP_000132614.1
• Coordinates: 4305050..4305391 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	timR
Locus tag	-
Coordinates	4304979..4305055 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B79_RS21050 (4301591)	4301591..4303060	+	1470	WP_001387312.1	type I restriction-modification system subunit M	-
K8B79_RS21055 (4303060)	4303060..4304829	+	1770	WP_001513535.1	restriction endonuclease subunit S	-
- (4304979)	4304979..4305055	-	77	NuclAT_8	-	Antitoxin
- (4304979)	4304979..4305055	-	77	NuclAT_8	-	Antitoxin
- (4304979)	4304979..4305055	-	77	NuclAT_8	-	Antitoxin
- (4304979)	4304979..4305055	-	77	NuclAT_8	-	Antitoxin
- (4304979)	4304979..4305055	-	77	NuclAT_9	-	Antitoxin
- (4304979)	4304979..4305055	-	77	NuclAT_9	-	Antitoxin
- (4304979)	4304979..4305055	-	77	NuclAT_9	-	Antitoxin
- (4304979)	4304979..4305055	-	77	NuclAT_9	-	Antitoxin
K8B79_RS21060 (4305050)	4305050..4305391	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
K8B79_RS26560 (4305438)	4305438..4306601	-	1164	WP_224153787.1	DUF1524 domain-containing protein	-
K8B79_RS26565 (4306655)	4306655..4307531	-	877	Protein_4154	DUF262 domain-containing protein	-
K8B79_RS21075 (4307937)	4307937..4308857	-	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
K8B79_RS21080 (4309042)	4309042..4310322	+	1281	WP_001338077.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4289367..4305391	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T215252 WP_000132614.1 NZ_CP082824:4305050-4305391 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T215252 NZ_CP110619:1014870-1015304 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT215252 NZ_CP082824:c4305055-4304979 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin