Detailed information of TA system TA215248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3913496..3914190
• Replicon: chromosome
• Accession: NZ_CP082824
• Organism: Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: K8B79_RS19190
• Protein ID: WP_001263500.1
• Coordinates: 3913496..3913894 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: K8B79_RS19195
• Protein ID: WP_000554758.1
• Coordinates: 3913897..3914190 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B79_RS19165 (3908861)	3908861..3909319	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
K8B79_RS19170 (3909580)	3909580..3911037	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
K8B79_RS19175 (3911094)	3911094..3911708	-	615	WP_000602124.1	peptide chain release factor H	-
K8B79_RS19180 (3911705)	3911705..3912844	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
K8B79_RS19185 (3913034)	3913034..3913486	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
K8B79_RS19190 (3913496)	3913496..3913894	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
K8B79_RS19195 (3913897)	3913897..3914190	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
K8B79_RS19200 (3914242)	3914242..3915297	-	1056	WP_001226168.1	DNA polymerase IV	-
K8B79_RS19205 (3915368)	3915368..3916291	-	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
K8B79_RS19210 (3916294)	3916294..3917157	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
K8B79_RS19215 (3917170)	3917170..3917886	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
K8B79_RS19220 (3917906)	3917906..3918373	-	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T215248 WP_001263500.1 NZ_CP082824:c3913894-3913496 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T215248 NZ_CP110619:c754996-754688 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT215248 WP_000554758.1 NZ_CP082824:c3914190-3913897 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT215248 NZ_CP110619:c755228-754983 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3