Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2614210..2614772 Replicon chromosome
Accession NZ_CP082824
Organism Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag K8B79_RS12700 Protein ID WP_000605675.1
Coordinates 2614210..2614488 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag K8B79_RS12705 Protein ID WP_000781370.1
Coordinates 2614488..2614772 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B79_RS12675 (2609796) 2609796..2610227 - 432 WP_000152305.1 peroxiredoxin OsmC -
K8B79_RS12680 (2610572) 2610572..2610787 + 216 WP_000495766.1 biofilm-dependent modulation protein -
K8B79_RS12685 (2610889) 2610889..2611026 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
K8B79_RS12690 (2611183) 2611183..2612880 + 1698 WP_000433460.1 malate dehydrogenase -
K8B79_RS12695 (2613014) 2613014..2614024 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
K8B79_RS12700 (2614210) 2614210..2614488 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B79_RS12705 (2614488) 2614488..2614772 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
K8B79_RS12710 (2615001) 2615001..2615654 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
K8B79_RS12715 (2615647) 2615647..2616531 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
K8B79_RS12720 (2616544) 2616544..2619591 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T215245 WP_000605675.1 NZ_CP082824:2614210-2614488 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T215245 NZ_CP110619:716667-717062 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT215245 WP_000781370.1 NZ_CP082824:2614488-2614772 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT215245 NZ_CP110619:716413-716667 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References