Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1972806..1973308 Replicon chromosome
Accession NZ_CP082824
Organism Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag K8B79_RS09585 Protein ID WP_000767822.1
Coordinates 1973054..1973308 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag K8B79_RS09580 Protein ID WP_001259255.1
Coordinates 1972806..1973057 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B79_RS09555 (1967984) 1967984..1969051 - 1068 WP_001538920.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
K8B79_RS09560 (1969051) 1969051..1970121 - 1071 WP_001538918.1 histidinol-phosphate transaminase -
K8B79_RS09565 (1970118) 1970118..1971422 - 1305 WP_001445483.1 histidinol dehydrogenase -
K8B79_RS09570 (1971428) 1971428..1972327 - 900 WP_000131779.1 ATP phosphoribosyltransferase -
K8B79_RS09575 (1972473) 1972473..1972523 - 51 WP_001364200.1 his operon leader peptide -
K8B79_RS09580 (1972806) 1972806..1973057 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
K8B79_RS09585 (1973054) 1973054..1973308 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
K8B79_RS09590 (1973391) 1973391..1974215 + 825 WP_000754737.1 SDR family oxidoreductase -
K8B79_RS09595 (1974261) 1974261..1975190 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
K8B79_RS09600 (1975405) 1975405..1975467 + 63 WP_010723108.1 membrane protein YoeI -
K8B79_RS09605 (1975457) 1975457..1976815 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T215235 WP_000767822.1 NZ_CP082824:1973054-1973308 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T215235 NZ_CP110619:363476-363778 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT215235 WP_001259255.1 NZ_CP082824:1972806-1973057 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT215235 NZ_CP110619:363252-363479 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References