Detailed information of TA system TA215229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6147763..6148358
• Replicon: chromosome
• Accession: NZ_CP082823
• Organism: Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: K8B71_RS29770
• Protein ID: WP_003113526.1
• Coordinates: 6148080..6148358 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B71_RS29765
• Protein ID: WP_003133769.1
• Coordinates: 6147763..6148068 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B71_RS29745 (K8B71_29745)	6143153..6143743	+	591	WP_071536478.1	SLATT domain-containing protein	-
K8B71_RS29750 (K8B71_29750)	6143740..6145005	+	1266	WP_023094906.1	nucleotidyltransferase	-
K8B71_RS29755 (K8B71_29755)	6145089..6145988	-	900	WP_225932746.1	hypothetical protein	-
K8B71_RS29765 (K8B71_29765)	6147763..6148068	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
K8B71_RS29770 (K8B71_29770)	6148080..6148358	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B71_RS34640	6148411..6148539	-	129	Protein_5903	integrase	-
K8B71_RS29775 (K8B71_29775)	6148687..6150915	+	2229	WP_003133778.1	TonB-dependent receptor	-
K8B71_RS29780 (K8B71_29780)	6150986..6151633	-	648	WP_003095021.1	carbonate dehydratase	-
K8B71_RS29785 (K8B71_29785)	6151695..6152933	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T215229 WP_003113526.1 NZ_CP082823:c6148358-6148080 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T215229 NZ_CP110611:1991652-1991840 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT215229 WP_003133769.1 NZ_CP082823:c6148068-6147763 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT215229 NZ_CP110611:1991370-1991627 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: V6ALY3