215226

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 5254614..5255295 Replicon chromosome
Accession NZ_CP082823
Organism Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag K8B71_RS25375 Protein ID WP_015503432.1
Coordinates 5254930..5255295 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K8B71_RS25370 Protein ID WP_004364117.1
Coordinates 5254614..5254937 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B71_RS25340 (K8B71_25340) 5250390..5250967 + 578 Protein_5026 plasmid pRiA4b ORF-3 family protein -
K8B71_RS34550 5251333..5251728 - 396 Protein_5027 hypothetical protein -
K8B71_RS34555 5252988..5253992 - 1005 Protein_5029 tyrosine-type recombinase/integrase -
K8B71_RS25360 (K8B71_25360) 5253992..5254384 - 393 Protein_5030 hypothetical protein -
K8B71_RS25365 (K8B71_25365) 5254379..5254498 + 120 Protein_5031 IS5/IS1182 family transposase -
K8B71_RS25370 (K8B71_25370) 5254614..5254937 - 324 WP_004364117.1 XRE family transcriptional regulator Antitoxin
K8B71_RS25375 (K8B71_25375) 5254930..5255295 - 366 WP_015503432.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B71_RS25380 (K8B71_25380) 5255559..5255798 - 240 WP_044069615.1 hypothetical protein -
K8B71_RS25385 (K8B71_25385) 5256005..5256277 + 273 WP_004364121.1 hypothetical protein -
K8B71_RS25390 (K8B71_25390) 5256308..5256733 - 426 WP_003116492.1 VOC family protein -
K8B71_RS25395 (K8B71_25395) 5256834..5257718 + 885 WP_004364122.1 LysR substrate-binding domain-containing protein -
K8B71_RS25400 (K8B71_25400) 5257691..5258644 - 954 WP_003085661.1 LysR substrate-binding domain-containing protein -
K8B71_RS25405 (K8B71_25405) 5258865..5259299 + 435 WP_003158601.1 RidA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5221171..5256733 35562


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

HigA2-like, helix-turn-helix domain

(12-86)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13798.14 Da        Isoelectric Point: 4.8219

>T215226 WP_015503432.1 NZ_CP082823:c5255295-5254930 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Download         Length: 366 bp

>T215226 NZ_CP110611:806184-806393 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11896.73 Da        Isoelectric Point: 6.4751

>AT215226 WP_004364117.1 NZ_CP082823:c5254937-5254614 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GHLEVIARFPDGAVKISNFADIEEAGA

Download         Length: 324 bp

>AT215226 NZ_CP110611:805884-806177 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References