Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5493859..5494454 Replicon chromosome
Accession NZ_CP082822
Organism Pseudomonas aeruginosa strain SCAID WND1-2021 (9/195)

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag K8B69_RS26110 Protein ID WP_003113526.1
Coordinates 5494176..5494454 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K8B69_RS26105 Protein ID WP_003113527.1
Coordinates 5493859..5494164 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B69_RS26085 (K8B69_26085) 5489935..5490192 + 258 WP_010793759.1 ATP-binding protein -
K8B69_RS26090 (K8B69_26090) 5490568..5491431 - 864 WP_010793758.1 integrase domain-containing protein -
K8B69_RS26095 (K8B69_26095) 5492033..5493175 - 1143 WP_010793757.1 STY4528 family pathogenicity island replication protein -
K8B69_RS26105 (K8B69_26105) 5493859..5494164 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
K8B69_RS26110 (K8B69_26110) 5494176..5494454 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B69_RS34005 5494507..5494635 - 129 Protein_5179 integrase -
K8B69_RS26115 (K8B69_26115) 5494783..5497011 + 2229 WP_003099265.1 TonB-dependent receptor -
K8B69_RS26120 (K8B69_26120) 5497081..5497728 - 648 WP_003095021.1 carbonate dehydratase -
K8B69_RS26125 (K8B69_26125) 5497790..5499028 - 1239 WP_010793756.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T215218 WP_003113526.1 NZ_CP082822:c5494454-5494176 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T215218 NZ_CP110534:c4600305-4599934 [Enterobacter roggenkampii]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GAATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTGATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATCCTAGCTACAGCGCAGAGCAGAAAATGTCCGTTGGTTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT215218 WP_003113527.1 NZ_CP082822:c5494164-5493859 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT215218 NZ_CP110534:c4600550-4600308 [Enterobacter roggenkampii]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAACCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGCGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References