Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1618992..1619554 Replicon chromosome
Accession NC_007946
Organism Escherichia coli UTI89

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag UTI89_RS08200 Protein ID WP_000605675.1
Coordinates 1619276..1619554 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag UTI89_RS08195 Protein ID WP_000781370.1
Coordinates 1618992..1619276 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UTI89_RS08180 1614173..1617220 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
UTI89_RS08185 1617233..1618117 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
UTI89_RS08190 1618110..1618763 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
UTI89_RS08195 1618992..1619276 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
UTI89_RS08200 1619276..1619554 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
UTI89_RS08205 1619740..1620750 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
UTI89_RS08210 1620884..1622581 - 1698 WP_000433462.1 malate dehydrogenase -
UTI89_RS08215 1622737..1622874 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
UTI89_RS08220 1622976..1623191 - 216 WP_000495766.1 biofilm-dependent modulation protein -
UTI89_RS08225 1623536..1623967 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T21521 WP_000605675.1 NC_007946:c1619554-1619276 [Escherichia coli UTI89]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T21521 NC_007946:c1619554-1619276 [Escherichia coli UTI89]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT21521 WP_000781370.1 NC_007946:c1619276-1618992 [Escherichia coli UTI89]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT21521 NC_007946:c1619276-1618992 [Escherichia coli UTI89]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References