Detailed information of TA system TA215206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5198815..5199496
• Replicon: chromosome
• Accession: NZ_CP082821
• Organism: Pseudomonas aeruginosa strain SCAID PLC1-2021 (16/222)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K8B72_RS25045
• Protein ID: WP_015503432.1
• Coordinates: 5199131..5199496 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B72_RS25040
• Protein ID: WP_004364117.1
• Coordinates: 5198815..5199138 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B72_RS25010 (K8B72_25010)	5194591..5195168	+	578	Protein_4956	plasmid pRiA4b ORF-3 family protein	-
K8B72_RS34325	5195534..5195929	-	396	Protein_4957	hypothetical protein	-
K8B72_RS34330	5197189..5198193	-	1005	Protein_4959	tyrosine-type recombinase/integrase	-
K8B72_RS25030 (K8B72_25030)	5198193..5198585	-	393	Protein_4960	hypothetical protein	-
K8B72_RS25035 (K8B72_25035)	5198580..5198699	+	120	Protein_4961	IS5/IS1182 family transposase	-
K8B72_RS25040 (K8B72_25040)	5198815..5199138	-	324	WP_004364117.1	XRE family transcriptional regulator	Antitoxin
K8B72_RS25045 (K8B72_25045)	5199131..5199496	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B72_RS25050 (K8B72_25050)	5199760..5199999	-	240	WP_044069615.1	hypothetical protein	-
K8B72_RS25055 (K8B72_25055)	5200206..5200478	+	273	WP_004364121.1	hypothetical protein	-
K8B72_RS25060 (K8B72_25060)	5200509..5200934	-	426	WP_003116492.1	VOC family protein	-
K8B72_RS25065 (K8B72_25065)	5201035..5201919	+	885	WP_004364122.1	LysR substrate-binding domain-containing protein	-
K8B72_RS25070 (K8B72_25070)	5201892..5202845	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
K8B72_RS25075 (K8B72_25075)	5203066..5203500	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T215206 WP_015503432.1 NZ_CP082821:c5199496-5199131 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T215206 NZ_CP110533:c2962921-2962568 [Enterobacter quasiroggenkampii]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCGCTTCGCTTGATGATACGGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCATAGCA
ACATGAAAGAGTTACGTATTCAAAGTAAGGGTGATCCATTGCGGGCGTTTTTCGCCTTCGACCCAAACCGTACTGGCATC
ATGCTTTGTGCTGGCAATAAGGTCGGTAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGATCGGGAATTCAC
TCACTGGCTGAATACATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT215206 WP_004364117.1 NZ_CP082821:c5199138-5198815 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GHLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT215206 NZ_CP110533:c2962563-2962261 [Enterobacter quasiroggenkampii]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCTGAAGTTGTCGCCAGCGCTCAGGCAATAGCAACGGATAT
TTTGCTCAATATTCATCTGGCTGAGTTACGAGAGAAAGTACAAAAAACGCAAGTAGAGATGGCACAGGCTCTGGGTATCA
GGCAGCCTACCGTTGCGGTAATGGAAAAACCCGGGCGCGATTTGAAACTCTCTTCACTCAAGCGTTACGTTGAAGCTGCG
GGCGGAAAGCTGCGGCTTGATGTTGAGCTGCCAGATGGCTCGCACTATGAGTTTGTGCTGTAG