Detailed information of TA system TA215159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 167485..168212
• Replicon: plasmid unnamed
• Accession: NZ_CP082806
• Organism: Klebsiella pneumoniae strain SCAID PHRX1-2021 (13/97)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: K8B74_RS27405
• Protein ID: WP_011251285.1
• Coordinates: 167485..167796 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B74_RS27410
• Protein ID: WP_011251286.1
• Coordinates: 167793..168212 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B74_RS27365 (K8B74_27365)	162546..163061	-	516	WP_041937820.1	hypothetical protein	-
K8B74_RS27370 (K8B74_27370)	163169..163744	-	576	WP_074424927.1	hypothetical protein	-
K8B74_RS27375 (K8B74_27375)	163861..164817	-	957	WP_011251280.1	DsbA family protein	-
K8B74_RS27380 (K8B74_27380)	164877..165218	-	342	WP_011251281.1	hypothetical protein	-
K8B74_RS27385 (K8B74_27385)	165232..165543	-	312	WP_011251282.1	hypothetical protein	-
K8B74_RS27390 (K8B74_27390)	165560..166009	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
K8B74_RS27400 (K8B74_27400)	166843..167280	+	438	Protein_189	DDE-type integrase/transposase/recombinase	-
K8B74_RS27405 (K8B74_27405)	167485..167796	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
K8B74_RS27410 (K8B74_27410)	167793..168212	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
K8B74_RS27415 (K8B74_27415)	168359..169327	+	969	WP_074428168.1	IS5 family transposase	-
K8B74_RS27420 (K8B74_27420)	169399..169764	-	366	WP_048333448.1	hypothetical protein	-
K8B74_RS27425 (K8B74_27425)	169778..170566	-	789	WP_040217257.1	hypothetical protein	-
K8B74_RS27430 (K8B74_27430)	170587..171207	-	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
K8B74_RS27435 (K8B74_27435)	171626..172261	+	636	WP_223171879.1	hypothetical protein	-
K8B74_RS27440 (K8B74_27440)	172574..173044	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..217781	217781
	-	inside	IScluster/Tn	-	-	166011..178333	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T215159 WP_011251285.1 NZ_CP082806:167485-167796 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T215159 NZ_CP110515:c159812-159342 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTATCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGAATCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT215159 WP_011251286.1 NZ_CP082806:167793-168212 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT215159 NZ_CP110515:c160252-159809 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1