Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 418232..418959 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP082800 | ||
| Organism | Klebsiella pneumoniae strain SCAID TST1-2021 (15/226) | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | K8B70_RS27290 | Protein ID | WP_011251285.1 |
| Coordinates | 418648..418959 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | K8B70_RS27285 | Protein ID | WP_011251286.1 |
| Coordinates | 418232..418651 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K8B70_RS27255 (K8B70_27255) | 413347..413871 | - | 525 | WP_040217250.1 | hypothetical protein | - |
| K8B70_RS27260 (K8B70_27260) | 414184..414819 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| K8B70_RS27265 (K8B70_27265) | 415414..415857 | + | 444 | WP_040217253.1 | conjugative transfer protein MobI(A/C) | - |
| K8B70_RS27270 (K8B70_27270) | 415878..416666 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| K8B70_RS27275 (K8B70_27275) | 416680..417045 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| K8B70_RS27280 (K8B70_27280) | 417094..418062 | + | 969 | WP_072193960.1 | IS5 family transposase | - |
| K8B70_RS27285 (K8B70_27285) | 418232..418651 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| K8B70_RS27290 (K8B70_27290) | 418648..418959 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| K8B70_RS27295 (K8B70_27295) | 419164..419601 | - | 438 | Protein_442 | DDE-type integrase/transposase/recombinase | - |
| K8B70_RS27300 (K8B70_27300) | 419715..420053 | + | 339 | WP_023288266.1 | transposase | - |
| K8B70_RS27305 (K8B70_27305) | 420050..420397 | + | 348 | WP_040217144.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| K8B70_RS27310 (K8B70_27310) | 420446..421495 | + | 1050 | Protein_445 | IS66 family transposase | - |
| K8B70_RS27315 (K8B70_27315) | 421553..422521 | - | 969 | WP_077268206.1 | IS5 family transposase | - |
| K8B70_RS27320 (K8B70_27320) | 422816..422938 | - | 123 | Protein_447 | IS110 family transposase | - |
| K8B70_RS27325 (K8B70_27325) | 423578..423782 | - | 205 | Protein_448 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | formA | mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / pla | 1..427807 | 427807 | |
| - | inside | IScluster/Tn | - | - | 408595..427306 | 18711 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T215141 WP_011251285.1 NZ_CP082800:c418959-418648 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T215141 NZ_CP110512:71875-72162 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT215141 WP_011251286.1 NZ_CP082800:c418651-418232 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT215141 NZ_CP110512:71627-71878 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|