Detailed information of TA system TA021513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 284004..284698
• Replicon: chromosome
• Accession: NC_007946
• Organism: Escherichia coli UTI89

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: UTI89_RS01335
• Protein ID: WP_001263500.1
• Coordinates: 284300..284698 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: UTI89_RS01330
• Protein ID: WP_000554758.1
• Coordinates: 284004..284297 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UTI89_RS01310	279540..280037	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
UTI89_RS29155	280120..280278	-	159	WP_014639450.1	hypothetical protein	-
UTI89_RS01315	280357..282069	-	1713	Protein_252	flagellar biosynthesis protein FlhA	-
UTI89_RS01320	282041..282826	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
UTI89_RS01325	282897..283952	+	1056	WP_001226168.1	DNA polymerase IV	-
UTI89_RS01330	284004..284297	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
UTI89_RS01335	284300..284698	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
UTI89_RS01340	284708..285160	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
UTI89_RS01345	285350..286489	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
UTI89_RS01350	286486..287100	+	615	WP_000602123.1	peptide chain release factor H	-
UTI89_RS01355	287157..288614	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
UTI89_RS01360	288875..289333	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	264479..285160	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T21513 WP_001263500.1 NC_007946:284300-284698 [Escherichia coli UTI89]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T21513 NC_007946:284300-284698 [Escherichia coli UTI89]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT21513 WP_000554758.1 NC_007946:284004-284297 [Escherichia coli UTI89]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT21513 NC_007946:284004-284297 [Escherichia coli UTI89]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3