Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 358605..359251 Replicon chromosome
Accession NZ_CP082799
Organism Klebsiella pneumoniae strain SCAID TST1-2021 (15/226)

Toxin (Protein)


Gene name higB Uniprot ID A0A486RBU5
Locus tag K8B70_RS01665 Protein ID WP_032410616.1
Coordinates 358605..358952 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag K8B70_RS01670 Protein ID WP_002920557.1
Coordinates 358952..359251 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B70_RS01655 (354531) 354531..355964 + 1434 WP_002920564.1 glycogen synthase GlgA -
K8B70_RS01660 (355982) 355982..358429 + 2448 WP_002920561.1 glycogen phosphorylase -
K8B70_RS01665 (358605) 358605..358952 + 348 WP_032410616.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B70_RS01670 (358952) 358952..359251 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
K8B70_RS01675 (359314) 359314..360822 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
K8B70_RS01680 (361027) 361027..361356 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
K8B70_RS01685 (361407) 361407..362237 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
K8B70_RS01690 (362287) 362287..363045 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13545.55 Da        Isoelectric Point: 5.6802

>T215121 WP_032410616.1 NZ_CP082799:358605-358952 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATILILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFEPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T215121 NZ_CP110473:c3609792-3609439 [Pantoea anthophila]
ATGTGGGATATCGATACAACCCATCTCTTTGACGAGTGGTTTGAGACCCAGACTGAGGCGCTGAAGGAGGATATGCTCGC
CGCGATGATTATCCTTTCCGAGTATGGTCCTCAGTTAGGAAGGCCGTATGCTGATACGGTAGACGACTCTGACTTTCCAA
ATATGAAAGAATTAAGAGTGCAGCATCAGGGCAATCCGATTCGGGCCTTCTTCGCGTTTGATCCTGCTCGCCGTGGCATT
GTGTTATGTGCTGGCGATAAAACCGGGGTTAAAGAAAAAAGATTCTATAAAGAGATGATCAAACTGGCAGATGCTGAATT
CCGCAAACACCTGAAAAGAGGGGAAAATGGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT215121 WP_002920557.1 NZ_CP082799:358952-359251 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT215121 NZ_CP110473:c3609446-3609147 [Pantoea anthophila]
ATGGCTAATTTAAAAGAGCTGATGGCAAAGCAAAGCCCGGAAAGTCGTGAGCGTATTGCCGAAAAAGTGGACGAAATGCG
GCAGGTTATTGCCCTGAACATGTTGCGGGAAGAACTTAACCTATCCCAGACAGAGATGGCGCGAGCCATGGGGGTGAAGC
AGCCAACCATTGCCAGAATGGAGCAGGCTGACAACGATCCACGCCTTTCTACGCTGAAGCGTTACGTTACAGCTCTGGGA
GGCGAGATAAGTATTGATGTCAGACTGCCGACAGGAAAAAGAATTGCTTTTGATCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486RBU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References