Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 332419..333005 Replicon chromosome
Accession NZ_CP082799
Organism Klebsiella pneumoniae strain SCAID TST1-2021 (15/226)

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag K8B70_RS01555 Protein ID WP_032421870.1
Coordinates 332637..333005 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag K8B70_RS01550 Protein ID WP_004174006.1
Coordinates 332419..332640 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B70_RS01530 (328576) 328576..329502 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K8B70_RS01535 (329499) 329499..330776 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
K8B70_RS01540 (330773) 330773..331540 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K8B70_RS01545 (331542) 331542..332255 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
K8B70_RS01550 (332419) 332419..332640 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K8B70_RS01555 (332637) 332637..333005 + 369 WP_032421870.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K8B70_RS01560 (333278) 333278..334594 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
K8B70_RS01565 (334701) 334701..335588 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
K8B70_RS01570 (335585) 335585..336430 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
K8B70_RS01575 (336432) 336432..337502 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 329499..338239 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13570.96 Da        Isoelectric Point: 8.6410

>T215120 WP_032421870.1 NZ_CP082799:332637-333005 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAMSRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T215120 NZ_CP110473:2951008-2951223 [Pantoea anthophila]
ATGAACAAAACACTGACAAAAACTGATTATTTGATGCGACTGCGTCGCTGTCGCTCCCTGGATACCCTGGAGCGGGTGAT
CGAAAAGAATAAGTATGAATTACCGGAAGATGAATTAGCGGTATTCTATTCGGCAGCTGACCATCGTCTGGCCGAGCTGA
CGATGAATAAGCTCTACGATAAAGTACCGGGTTCGGTCTGGAAGTTTGTCCGCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT215120 WP_004174006.1 NZ_CP082799:332419-332640 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT215120 NZ_CP110473:2950606-2950983 [Pantoea anthophila]
ATGGACGAATACTCACCAAAACGGCATGATATTGCCCAGCTGAAATACCTGTGTGAGAGCCTGTTTGACGACAGCATGGC
AACATTGACTGACAGCCACCATGGCTGGGTAAATGATCCCACTTCTGAAAGCAATCTGCACCTGAACGATCTGATTGAAC
ATATCGCTTCGTTCACCATGAATTACAAAATTAAACACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGTTATTCAGTAACTACGGTATCAATAATCCTGATCTTCAGCGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References