Detailed information of TA system TA215098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 341883..342529
• Replicon: chromosome
• Accession: NZ_CP082796
• Organism: Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A231WWF9
• Locus tag: K8B77_RS01610
• Protein ID: WP_004174016.1
• Coordinates: 341883..342230 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B77_RS01615
• Protein ID: WP_223367845.1
• Coordinates: 342284..342529 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B77_RS01600 (337809)	337809..339242	+	1434	WP_032445350.1	glycogen synthase GlgA	-
K8B77_RS01605 (339260)	339260..341707	+	2448	WP_002920561.1	glycogen phosphorylase	-
K8B77_RS01610 (341883)	341883..342230	+	348	WP_004174016.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B77_RS01615 (342284)	342284..342529	+	246	WP_223367845.1	helix-turn-helix transcriptional regulator	Antitoxin
K8B77_RS01620 (342592)	342592..344100	-	1509	WP_019704656.1	glycerol-3-phosphate dehydrogenase	-
K8B77_RS01625 (344305)	344305..344634	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
K8B77_RS01630 (344685)	344685..345515	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
K8B77_RS01635 (345565)	345565..346323	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-66)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13518.55 Da
• Isoelectric Point: 6.2327

>T215098 WP_004174016.1 NZ_CP082796:341883-342230 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T215098 NZ_CP110404:c3160224-3159973 [Shigella sonnei]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8717.13 Da
• Isoelectric Point: 8.6165

>AT215098 WP_223367845.1 NZ_CP082796:342284-342529 [Klebsiella pneumoniae]
IAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALGGELSINVTLPTGKKVSFN
L

Nucleotide

• Length: 246 bp

>AT215098 NZ_CP110404:c3159976-3159722 [Shigella sonnei]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A231WWF9