Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 315698..316284 Replicon chromosome
Accession NZ_CP082796
Organism Klebsiella pneumoniae strain SCAID PHRX2-2021 (20/245)

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag K8B77_RS01500 Protein ID WP_002920800.1
Coordinates 315916..316284 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag K8B77_RS01495 Protein ID WP_004174006.1
Coordinates 315698..315919 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B77_RS01475 (311855) 311855..312781 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K8B77_RS01480 (312778) 312778..314055 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
K8B77_RS01485 (314052) 314052..314819 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K8B77_RS01490 (314821) 314821..315534 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
K8B77_RS01495 (315698) 315698..315919 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K8B77_RS01500 (315916) 315916..316284 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K8B77_RS01505 (316557) 316557..317873 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
K8B77_RS01510 (317980) 317980..318867 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
K8B77_RS01515 (318864) 318864..319709 + 846 WP_004174009.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
K8B77_RS01520 (319711) 319711..320781 + 1071 WP_032445345.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 314052..321518 7466


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T215097 WP_002920800.1 NZ_CP082796:315916-316284 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T215097 NZ_CP110404:c3159976-3159722 [Shigella sonnei]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT215097 WP_004174006.1 NZ_CP082796:315698-315919 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT215097 NZ_CP110404:c3160224-3159973 [Shigella sonnei]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References