Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1901568..1902165 Replicon chromosome
Accession NZ_CP082791
Organism Klebsiella pneumoniae strain KP4823

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KCV57_RS09255 Protein ID WP_004142563.1
Coordinates 1901848..1902165 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KCV57_RS09250 Protein ID WP_004142561.1
Coordinates 1901568..1901855 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCV57_RS09220 (1897649) 1897649..1897897 + 249 WP_002893037.1 DUF1158 domain-containing protein -
KCV57_RS09225 (1897915) 1897915..1898256 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KCV57_RS09230 (1898287) 1898287..1899402 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
KCV57_RS09235 (1899581) 1899581..1900162 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
KCV57_RS09240 (1900162) 1900162..1900530 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
KCV57_RS09245 (1900650) 1900650..1901303 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KCV57_RS09250 (1901568) 1901568..1901855 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KCV57_RS09255 (1901848) 1901848..1902165 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KCV57_RS09260 (1902350) 1902350..1903393 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
KCV57_RS09265 (1904060) 1904060..1904926 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
KCV57_RS09270 (1905035) 1905035..1906462 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T215076 WP_004142563.1 NZ_CP082791:c1902165-1901848 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215076 NZ_CP110398:2334570-2334673 [Shigella sonnei]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT215076 WP_004142561.1 NZ_CP082791:c1901855-1901568 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215076 NZ_CP110398:c2334674-2334440 [Shigella sonnei]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References