Detailed information of TA system TA215053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 26070..26821
• Replicon: plasmid pADAP
• Accession: NZ_CP082788
• Organism: Serratia entomophila strain A1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KHA73_RS23995
• Protein ID: WP_169558228.1
• Coordinates: 26510..26821 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KHA73_RS23990
• Protein ID: WP_179917514.1
• Coordinates: 26070..26471 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHA73_RS23960 (KHA73_23965)	21989..22294	-	306	WP_010895858.1	plasmid mobilization protein MobA	-
KHA73_RS23965 (KHA73_23970)	22563..22862	+	300	WP_169558233.1	mobilization protein MobC	-
KHA73_RS23970 (KHA73_23975)	23034..23345	-	312	WP_234591460.1	DUF5983 family protein	-
KHA73_RS23975 (KHA73_23980)	23392..24210	-	819	WP_169558232.1	DUF932 domain-containing protein	-
KHA73_RS23980 (KHA73_23985)	24677..24826	-	150	WP_169558229.1	hypothetical protein	-
KHA73_RS23985 (KHA73_23990)	24885..25337	-	453	WP_010895853.1	Sea42	-
KHA73_RS23990 (KHA73_23995)	26070..26471	-	402	WP_179917514.1	helix-turn-helix domain-containing protein	Antitoxin
KHA73_RS23995 (KHA73_24000)	26510..26821	-	312	WP_169558228.1	type II toxin-antitoxin system HigB family toxin	Toxin
KHA73_RS24000 (KHA73_24005)	26934..27662	-	729	WP_010895850.1	plasmid SOS inhibition protein A	-
KHA73_RS24005 (KHA73_24010)	27659..28123	-	465	WP_010895849.1	conjugation system SOS inhibitor PsiB	-
KHA73_RS24010 (KHA73_24015)	28165..30207	-	2043	WP_169558227.1	ParB/RepB/Spo0J family partition protein	-
KHA73_RS24015 (KHA73_24020)	30971..31171	-	201	WP_169558226.1	hypothetical protein	-
KHA73_RS24020 (KHA73_24025)	31212..31717	-	506	Protein_33	antirestriction protein ArdA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..153828	153828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12259.23 Da
• Isoelectric Point: 10.1438

>T215053 WP_169558228.1 NZ_CP082788:c26821-26510 [Serratia entomophila]
VHVISRQPFNEAAGKYPNSALALLDLMRVLEKKTFNSPDEMKRAIPSLDNFKYRKKWWIIDVSGNTLRLMAFIDFEKQKV
FVKHIATHAEYDKLTKYYREHKE

Nucleotide

• Length: 312 bp

>T215053 NZ_CP110376:1887919-1888443 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAACTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14735.87 Da
• Isoelectric Point: 4.6655

>AT215053 WP_179917514.1 NZ_CP082788:c26471-26070 [Serratia entomophila]
MIATFANAVKCIPLMTGDKSEAEYKKALELIEYLVDRDELSNPLFEPLAAKITEYENSAPEFEAFNRRLAEIPTGIAALR
TLMDQHGLKAADLEDELGSKSNVSNILNGRRALTVQHIKALATRFDVPADVFI

Nucleotide

• Length: 402 bp

>AT215053 NZ_CP110376:1887644-1887922 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA