Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3786909..3787506 Replicon chromosome
Accession NZ_CP082785
Organism Klebsiella quasipneumoniae strain MMCC7

Toxin (Protein)


Gene name higB Uniprot ID A0A3T0QUS6
Locus tag K7176_RS18595 Protein ID WP_017899012.1
Coordinates 3787189..3787506 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A3T0QUV3
Locus tag K7176_RS18590 Protein ID WP_017899013.1
Coordinates 3786909..3787196 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7176_RS18560 (3782996) 3782996..3783244 + 249 WP_002893037.1 DUF1158 domain-containing protein -
K7176_RS18565 (3783262) 3783262..3783603 - 342 WP_004202492.1 RamA family antibiotic efflux transcriptional regulator -
K7176_RS18570 (3783634) 3783634..3784749 - 1116 WP_025999133.1 MBL fold metallo-hydrolase -
K7176_RS18575 (3784929) 3784929..3785510 + 582 WP_017899014.1 TetR/AcrR family transcriptional regulator -
K7176_RS18580 (3785510) 3785510..3785878 + 369 WP_004202487.1 MmcQ/YjbR family DNA-binding protein -
K7176_RS18585 (3785998) 3785998..3786651 + 654 WP_004202485.1 oxygen-insensitive NAD(P)H nitroreductase -
K7176_RS18590 (3786909) 3786909..3787196 - 288 WP_017899013.1 helix-turn-helix transcriptional regulator Antitoxin
K7176_RS18595 (3787189) 3787189..3787506 - 318 WP_017899012.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K7176_RS18600 (3787691) 3787691..3788734 - 1044 WP_094334190.1 DUF2157 domain-containing protein -
K7176_RS18605 (3789267) 3789267..3790136 - 870 WP_017900842.1 helix-turn-helix transcriptional regulator -
K7176_RS18610 (3790245) 3790245..3791672 + 1428 WP_004202473.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-87)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12185.52 Da        Isoelectric Point: 11.2767

>T215035 WP_017899012.1 NZ_CP082785:c3787506-3787189 [Klebsiella quasipneumoniae]
MFRMVVHVDVKKELQALPAIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQNKTLFLLRVF
VKKTQKTPLSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T215035 NZ_CP110372:3433927-3434310 [Proteus mirabilis]
ATGATATGGGTTAGCGCACAGGAAGTTATCGCTTTCCATGATCGTATATTACAACGTTTCCCAGGTGTGGCCGGTATGTC
CGATCCTGGCAGAGCGGAAGCTCTTATTTACCGGGTGCAAAACCGTAAACATTATGAAGGCATTACAGATGTGTTTGAAC
TGGCAGCAACTTATTGGGTCGCTATCGCTCGAGGGCACATATTTAATGACGGTAATAAACGTACCGCATTTTTTGTGACC
ATGACATTTCTCTATCGTAATGGCATCAGGATCCGCGATACTGGCAATATGCTGGAAAACTTAACAGTAGAAGCAGCTAC
AGGTGAGAAAACCGTCGACCAGTTAGCAAAACATCTACAAAACTTGGTAGAGAAAACTAACTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10840.52 Da        Isoelectric Point: 10.2213

>AT215035 WP_017899013.1 NZ_CP082785:c3787196-3786909 [Klebsiella quasipneumoniae]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQHRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRTSLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT215035 NZ_CP110372:3433709-3433930 [Proteus mirabilis]
ATGCGTACATATACCTCGACACAAGCCCGAGCCAATATTTCAGAAGTATTAGATATTGCGACCCACGGTGAACCTGTTGA
AATCACCCGCAGAGACGGTTGTTCAGCGGTTGTTATCAGTAAAACTGAATTTGAGTCATACCAGAATGCTAAGCTAGATG
CAGAGTTTGATATGATGATGCAACGTCATGGACATACCGTAGAGGCACTGACAAATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0QUS6

Antitoxin

Source ID Structure
AlphaFold DB A0A3T0QUV3

References