Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3873323..3874050 Replicon chromosome
Accession NZ_CP082777
Organism Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag K8B91_RS18610 Protein ID WP_000547555.1
Coordinates 3873739..3874050 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag K8B91_RS18605 Protein ID WP_000126293.1
Coordinates 3873323..3873742 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B91_RS18590 (3869486) 3869486..3871738 - 2253 WP_001107659.1 carbamoyltransferase HypF -
K8B91_RS18595 (3871891) 3871891..3872418 - 528 WP_001078782.1 electron transport protein HydN -
K8B91_RS18600 (3872803) 3872803..3873231 + 429 WP_000536064.1 hypothetical protein -
K8B91_RS18605 (3873323) 3873323..3873742 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
K8B91_RS18610 (3873739) 3873739..3874050 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
K8B91_RS18615 (3874213) 3874213..3874968 - 756 WP_001305268.1 hypothetical protein -
K8B91_RS18620 (3875011) 3875011..3875481 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
K8B91_RS18625 (3875474) 3875474..3875884 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
K8B91_RS18630 (3875881) 3875881..3876648 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
K8B91_RS18635 (3876648) 3876648..3877190 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
K8B91_RS18640 (3877200) 3877200..3878909 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T215013 WP_000547555.1 NZ_CP082777:c3874050-3873739 [Escherichia coli O25:H1]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T215013 NZ_CP110365:509157-509507 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT215013 WP_000126293.1 NZ_CP082777:c3873742-3873323 [Escherichia coli O25:H1]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT215013 NZ_CP110365:508871-509152 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References