Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2573467..2574029 Replicon chromosome
Accession NZ_CP082777
Organism Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag K8B91_RS12610 Protein ID WP_000605675.1
Coordinates 2573751..2574029 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag K8B91_RS12605 Protein ID WP_000781370.1
Coordinates 2573467..2573751 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B91_RS12590 (2568648) 2568648..2571695 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
K8B91_RS12595 (2571708) 2571708..2572592 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
K8B91_RS12600 (2572585) 2572585..2573238 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
K8B91_RS12605 (2573467) 2573467..2573751 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
K8B91_RS12610 (2573751) 2573751..2574029 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B91_RS12615 (2574215) 2574215..2575225 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
K8B91_RS12620 (2575359) 2575359..2577056 - 1698 WP_000433460.1 malate dehydrogenase -
K8B91_RS12625 (2577213) 2577213..2577350 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
K8B91_RS12630 (2577452) 2577452..2577667 - 216 WP_000495766.1 biofilm-dependent modulation protein -
K8B91_RS12635 (2578012) 2578012..2578443 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T215001 WP_000605675.1 NZ_CP082777:c2574029-2573751 [Escherichia coli O25:H1]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T215001 NZ_CP110362:c467212-466898 [Dickeya dadantii]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGCCATTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
ACAGTTGAATCGTCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCCTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACGCACGAGCTGGCAAGCATCCCTGTGCAGGCCCTGGGG
GCTGTGTTTTGTGATGCGTCGCCGTACCGTTCTCAGGTAAAGGCCGCAATAGACTTCCTCATTGACGGCATTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT215001 WP_000781370.1 NZ_CP082777:c2573751-2573467 [Escherichia coli O25:H1]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT215001 NZ_CP110362:c467451-467215 [Dickeya dadantii]
ATGACCGCGAAACAACGTAACACGCAGAGCGTGACCATGACGGTAGAGCGTGCCTTGCTGGTCCGTGCGCGTGAAGCGGG
GATTAACCTGAGTGCTACCCTGACAGCCGCTCTGGATGCAGAGCTTCGCCACCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAATCCATCGACGCATTAAATCGTTTTCATGATGAACACGGCTGTTTCAGCGATGAATACCTGACATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References