Detailed information of TA system TA021500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 207787..208350
• Replicon: plasmid plasmid1
• Accession: NC_007901
• Organism: Albidiferax ferrireducens T118 (Rhodoferax ferrireducens T118)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q21Q09
• Locus tag: RFER_RS22585
• Protein ID: WP_011458603.1
• Coordinates: 208072..208350 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RFER_RS22580
• Protein ID: WP_011458604.1
• Coordinates: 207787..208062 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RFER_RS22525	202983..203297	-	315	WP_011458613.1	hypothetical protein	-
RFER_RS22530	203307..203903	-	597	WP_011458612.1	hypothetical protein	-
RFER_RS22535	203916..204236	-	321	WP_011458611.1	hypothetical protein	-
RFER_RS23750	204246..204647	-	402	WP_011458610.1	hypothetical protein	-
RFER_RS22540	204657..205100	-	444	WP_011458609.1	hypothetical protein	-
RFER_RS22545	205109..205435	-	327	WP_011458608.1	hypothetical protein	-
RFER_RS22550	205446..205631	-	186	WP_041793450.1	hypothetical protein	-
RFER_RS22555	205643..205888	-	246	WP_041793452.1	hypothetical protein	-
RFER_RS22560	205912..206694	-	783	WP_011458607.1	hypothetical protein	-
RFER_RS22565	206706..206954	-	249	WP_166485935.1	hypothetical protein	-
RFER_RS22575	207515..207784	-	270	WP_166485946.1	hypothetical protein	-
RFER_RS22580	207787..208062	-	276	WP_011458604.1	HigA family addiction module antidote protein	Antitoxin
RFER_RS22585	208072..208350	-	279	WP_011458603.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RFER_RS22590	208382..209071	-	690	WP_011458602.1	site-specific integrase	-
RFER_RS22595	209436..209708	-	273	WP_166485936.1	hypothetical protein	-
RFER_RS22600	209761..210000	-	240	WP_011458601.1	hypothetical protein	-
RFER_RS22605	210008..210511	-	504	WP_041793461.1	hypothetical protein	-
RFER_RS23215	210588..210887	-	300	WP_049765775.1	hypothetical protein	-
RFER_RS22615	210875..211540	-	666	WP_011458598.1	hypothetical protein	-
RFER_RS22620	211928..212212	-	285	WP_011458597.1	hypothetical protein	-
RFER_RS22625	212209..212976	-	768	WP_011458596.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..257447	257447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10502.05 Da
• Isoelectric Point: 10.1485

>T21500 WP_011458603.1 NC_007901:c208350-208072 [Rhodoferax ferrireducens T118]
MIKTFRHKGLERFFREGTKAGIQAAHANRLRLQLLALDQAVRPEDLSAPAWGLHPLKGDMKGHWAVTVNGNWRMVFAFDG
TDAVLVDYRDYH

Nucleotide

• Length: 279 bp

>T21500 NC_007901:c208350-208072 [Rhodoferax ferrireducens T118]
ATGATAAAGACTTTCCGGCACAAGGGATTGGAACGCTTTTTCCGTGAAGGCACCAAGGCGGGCATCCAAGCTGCGCATGC
AAACCGACTGCGGCTGCAACTGTTGGCGCTGGATCAGGCTGTAAGGCCTGAAGACCTGTCCGCGCCAGCATGGGGGCTGC
ATCCACTGAAGGGTGACATGAAGGGCCATTGGGCTGTGACCGTAAACGGGAATTGGCGGATGGTCTTTGCTTTTGACGGC
ACTGATGCCGTGTTGGTTGATTACCGTGACTACCACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10080.69 Da
• Isoelectric Point: 10.0899

>AT21500 WP_011458604.1 NC_007901:c208062-207787 [Rhodoferax ferrireducens T118]
MTRMHNPPHPGEVLREYLGDTTITEAAMKLGVNRVTLTRIVTGVSGISADMAYRLSAAFGTSAELWAGMQMQYDLWQAGK
VKRPRIERMAA

Nucleotide

• Length: 276 bp

>AT21500 NC_007901:c208062-207787 [Rhodoferax ferrireducens T118]
ATGACTCGAATGCACAATCCACCGCACCCAGGCGAAGTCCTGCGGGAATATTTGGGTGATACCACCATCACCGAAGCTGC
AATGAAGTTGGGTGTCAATCGCGTGACTTTGACCCGCATTGTGACGGGCGTATCCGGCATATCGGCTGACATGGCTTACC
GGCTCAGTGCTGCTTTTGGCACCAGTGCCGAACTGTGGGCGGGCATGCAGATGCAGTACGACCTCTGGCAAGCAGGAAAA
GTCAAACGCCCCCGGATTGAACGGATGGCGGCATGA

Structures

Toxin

• AlphaFold DB: Q21Q09