Detailed information of TA system TA214993

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 812412..812824
• Replicon: chromosome
• Accession: NZ_CP082777
• Organism: Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: K8B91_RS03905
• Protein ID: WP_000132630.1
• Coordinates: 812412..812753 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	812748..812824 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B91_RS03880 (807481)	807481..808761	-	1281	WP_001304526.1	DUF445 domain-containing protein	-
K8B91_RS03885 (808946)	808946..809866	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
K8B91_RS03890 (810094)	810094..810174	+	81	WP_020233658.1	hypothetical protein	-
K8B91_RS03895 (810272)	810272..811154	+	883	Protein_746	DUF262 domain-containing protein	-
K8B91_RS03900 (811202)	811202..812365	+	1164	WP_072202028.1	DUF1524 domain-containing protein	-
K8B91_RS03905 (812412)	812412..812753	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
- (812748)	812748..812824	+	77	NuclAT_6	-	Antitoxin
- (812748)	812748..812824	+	77	NuclAT_6	-	Antitoxin
- (812748)	812748..812824	+	77	NuclAT_6	-	Antitoxin
- (812748)	812748..812824	+	77	NuclAT_6	-	Antitoxin
- (812748)	812748..812824	+	77	NuclAT_7	-	Antitoxin
- (812748)	812748..812824	+	77	NuclAT_7	-	Antitoxin
- (812748)	812748..812824	+	77	NuclAT_7	-	Antitoxin
- (812748)	812748..812824	+	77	NuclAT_7	-	Antitoxin
K8B91_RS03910 (812974)	812974..814743	-	1770	WP_000110076.1	restriction endonuclease subunit S	-
K8B91_RS03915 (814743)	814743..816212	-	1470	WP_001315223.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	812412..820641	8229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T214993 WP_000132630.1 NZ_CP082777:c812753-812412 [Escherichia coli O25:H1]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T214993 NZ_CP110354:c3866877-3866467 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT214993 NZ_CP082777:812748-812824 [Escherichia coli O25:H1]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin