Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2929910..2930412 Replicon chromosome
Accession NZ_CP082776
Organism Escherichia coli O4:H5 strain CU532-9

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag K8B92_RS14280 Protein ID WP_000767828.1
Coordinates 2929910..2930164 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag K8B92_RS14285 Protein ID WP_001259255.1
Coordinates 2930161..2930412 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B92_RS14250 (2924925) 2924925..2925152 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
K8B92_RS14255 (2925166) 2925166..2926224 - 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
K8B92_RS14260 (2926403) 2926403..2927761 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
K8B92_RS14265 (2927751) 2927751..2927813 - 63 WP_010723108.1 membrane protein YoeI -
K8B92_RS14270 (2928028) 2928028..2928957 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
K8B92_RS14275 (2929003) 2929003..2929827 - 825 WP_000754740.1 SDR family oxidoreductase -
K8B92_RS14280 (2929910) 2929910..2930164 - 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
K8B92_RS14285 (2930161) 2930161..2930412 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
K8B92_RS14290 (2930695) 2930695..2930745 + 51 WP_001364200.1 his operon leader peptide -
K8B92_RS14295 (2930891) 2930891..2931790 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
K8B92_RS14300 (2931796) 2931796..2933100 + 1305 WP_001370829.1 histidinol dehydrogenase -
K8B92_RS14305 (2933097) 2933097..2934167 + 1071 WP_000108949.1 histidinol-phosphate transaminase -
K8B92_RS14310 (2934167) 2934167..2935234 + 1068 WP_000080129.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T214984 WP_000767828.1 NZ_CP082776:c2930164-2929910 [Escherichia coli O4:H5]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T214984 NZ_CP110353:3756319-3756897 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCAGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT214984 WP_001259255.1 NZ_CP082776:c2930412-2930161 [Escherichia coli O4:H5]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT214984 NZ_CP110353:3755676-3756230 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References