Detailed information of TA system TA214962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 3087007..3087509
• Replicon: chromosome
• Accession: NZ_CP082775
• Organism: Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: K8B89_RS15700
• Protein ID: WP_000767819.1
• Coordinates: 3087007..3087261 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: K8B89_RS15705
• Protein ID: WP_001259253.1
• Coordinates: 3087258..3087509 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B89_RS15675 (3082825)	3082825..3083283	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
K8B89_RS15680 (3083500)	3083500..3084858	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
K8B89_RS15685 (3084848)	3084848..3084910	-	63	WP_010723108.1	membrane protein YoeI	-
K8B89_RS15690 (3085125)	3085125..3086054	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
K8B89_RS15695 (3086100)	3086100..3086924	-	825	WP_000754737.1	SDR family oxidoreductase	-
K8B89_RS15700 (3087007)	3087007..3087261	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
K8B89_RS15705 (3087258)	3087258..3087509	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
K8B89_RS15710 (3087792)	3087792..3087842	+	51	WP_001364200.1	his operon leader peptide	-
K8B89_RS15715 (3087988)	3087988..3088887	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
K8B89_RS15720 (3088893)	3088893..3090197	+	1305	WP_001296211.1	histidinol dehydrogenase	-
K8B89_RS15725 (3090194)	3090194..3091264	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
K8B89_RS15730 (3091264)	3091264..3092331	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3082825..3083283	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T214962 WP_000767819.1 NZ_CP082775:c3087261-3087007 [Escherichia coli O25:H4]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T214962 NZ_CP110350:c1251837-1251556 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGTAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT214962 WP_001259253.1 NZ_CP082775:c3087509-3087258 [Escherichia coli O25:H4]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT214962 NZ_CP110350:c1252061-1251834 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3