Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1258281..1258960 Replicon chromosome
Accession NZ_CP082775
Organism Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag K8B89_RS05935 Protein ID WP_000057523.1
Coordinates 1258281..1258583 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag K8B89_RS05940 Protein ID WP_000806442.1
Coordinates 1258619..1258960 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B89_RS05910 (1253657) 1253657..1255309 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
K8B89_RS05915 (1255347) 1255347..1255850 - 504 WP_000667000.1 hypothetical protein -
K8B89_RS05920 (1255847) 1255847..1256647 - 801 WP_000439798.1 hypothetical protein -
K8B89_RS05925 (1256671) 1256671..1257150 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
K8B89_RS05930 (1257354) 1257354..1258148 - 795 WP_000365147.1 TraB/GumN family protein -
K8B89_RS05935 (1258281) 1258281..1258583 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B89_RS05940 (1258619) 1258619..1258960 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
K8B89_RS05945 (1259018) 1259018..1261522 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
K8B89_RS05950 (1261784) 1261784..1262716 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T214947 WP_000057523.1 NZ_CP082775:1258281-1258583 [Escherichia coli O25:H4]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T214947 NZ_CP110348:c155462-155181 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGTAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT214947 WP_000806442.1 NZ_CP082775:1258619-1258960 [Escherichia coli O25:H4]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT214947 NZ_CP110348:c155686-155459 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References