Detailed information of TA system TA214941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 167815..168417
• Replicon: chromosome
• Accession: NZ_CP082775
• Organism: Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: K8B89_RS00810
• Protein ID: WP_000897302.1
• Coordinates: 167815..168126 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B89_RS00815
• Protein ID: WP_000356397.1
• Coordinates: 168127..168417 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B89_RS00785 (163729)	163729..164328	+	600	WP_001296610.1	glucose-1-phosphatase	-
K8B89_RS00790 (164322)	164322..165194	+	873	WP_000920762.1	virulence factor BrkB family protein	-
K8B89_RS00795 (165191)	165191..165628	+	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
K8B89_RS00800 (165673)	165673..166614	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
K8B89_RS00805 (166678)	166678..167586	-	909	WP_001385591.1	alpha/beta hydrolase	-
K8B89_RS00810 (167815)	167815..168126	+	312	WP_000897302.1	hypothetical protein	Toxin
K8B89_RS00815 (168127)	168127..168417	+	291	WP_000356397.1	NadS family protein	Antitoxin
K8B89_RS00820 (168776)	168776..169054	+	279	WP_001296612.1	hypothetical protein	-
K8B89_RS00825 (169451)	169451..169669	+	219	WP_001251293.1	CopG family transcriptional regulator	-
K8B89_RS00830 (169854)	169854..170594	-	741	WP_000608806.1	hypothetical protein	-
K8B89_RS00835 (170619)	170619..171467	-	849	WP_001038650.1	hypothetical protein	-
K8B89_RS00840 (171757)	171757..171999	+	243	WP_001068514.1	CopG family transcriptional regulator	-
K8B89_RS00845 (172181)	172181..173110	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T214941 WP_000897302.1 NZ_CP082775:167815-168126 [Escherichia coli O25:H4]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T214941 NZ_CP110346:c5672471-5672193 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT214941 WP_000356397.1 NZ_CP082775:168127-168417 [Escherichia coli O25:H4]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT214941 NZ_CP110346:c5672181-5671876 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGGTATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: S1P416