Detailed information of TA system TA214921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1202035..1202729
• Replicon: chromosome
• Accession: NZ_CP082774
• Organism: Escherichia coli O16:H6 strain CU37RT-2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: K8B88_RS05745
• Protein ID: WP_001263500.1
• Coordinates: 1202331..1202729 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: K8B88_RS05740
• Protein ID: WP_000554758.1
• Coordinates: 1202035..1202328 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B88_RS05715 (1197473)	1197473..1197970	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
K8B88_RS05720 (1197965)	1197965..1198309	-	345	WP_125093574.1	hypothetical protein	-
K8B88_RS05725 (1198388)	1198388..1200100	-	1713	Protein_1106	flagellar biosynthesis protein FlhA	-
K8B88_RS05730 (1200072)	1200072..1200857	+	786	WP_061301231.1	putative lateral flagellar export/assembly protein LafU	-
K8B88_RS05735 (1200928)	1200928..1201983	+	1056	WP_001226168.1	DNA polymerase IV	-
K8B88_RS05740 (1202035)	1202035..1202328	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
K8B88_RS05745 (1202331)	1202331..1202729	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
K8B88_RS05750 (1202739)	1202739..1203191	+	453	WP_071778924.1	GNAT family N-acetyltransferase	-
K8B88_RS05755 (1203381)	1203381..1204520	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
K8B88_RS05760 (1204517)	1204517..1205131	+	615	WP_000602123.1	peptide chain release factor H	-
K8B88_RS05765 (1205188)	1205188..1206645	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
K8B88_RS05770 (1206906)	1206906..1207364	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1181150..1203191	22041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T214921 WP_001263500.1 NZ_CP082774:1202331-1202729 [Escherichia coli O16:H6]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T214921 NZ_CP110344:3089543-3089833 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT214921 WP_000554758.1 NZ_CP082774:1202035-1202328 [Escherichia coli O16:H6]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT214921 NZ_CP110344:3089247-3089546 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3