Detailed information of TA system TA214914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 175782..176384
• Replicon: chromosome
• Accession: NZ_CP082774
• Organism: Escherichia coli O16:H6 strain CU37RT-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: K8B88_RS00840
• Protein ID: WP_000897302.1
• Coordinates: 175782..176093 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B88_RS00845
• Protein ID: WP_000356397.1
• Coordinates: 176094..176384 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B88_RS00810 (170812)	170812..171597	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
K8B88_RS00815 (171696)	171696..172295	+	600	WP_001314324.1	glucose-1-phosphatase	-
K8B88_RS00820 (172289)	172289..173161	+	873	WP_000920762.1	virulence factor BrkB family protein	-
K8B88_RS00825 (173158)	173158..173595	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
K8B88_RS00830 (173640)	173640..174581	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
K8B88_RS00835 (174645)	174645..175553	-	909	WP_001331553.1	alpha/beta hydrolase	-
K8B88_RS00840 (175782)	175782..176093	+	312	WP_000897302.1	hypothetical protein	Toxin
K8B88_RS00845 (176094)	176094..176384	+	291	WP_000356397.1	NadS family protein	Antitoxin
K8B88_RS00850 (176743)	176743..177021	+	279	WP_001296612.1	hypothetical protein	-
K8B88_RS00855 (177417)	177417..177635	+	219	WP_001314326.1	CopG family transcriptional regulator	-
K8B88_RS00860 (177859)	177859..178788	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
K8B88_RS00865 (178785)	178785..179420	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
K8B88_RS00870 (179417)	179417..180319	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T214914 WP_000897302.1 NZ_CP082774:175782-176093 [Escherichia coli O16:H6]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T214914 NZ_CP110342:4087724-4088026 [Marinobacter sp. AN1]
ATGGCTGAAGTAATCTGGACGGAGCCCGCCCTTCAAGAACTGGATGCGATCGCTGAGTACATTGCTCTGGATAATCCTGC
AGCCGCAAGTCACCTGGTCCAAGAAGTTTTCGATAAGACCGAGCGCTTGGAAGATTTTCCCCAATCCGGACGGATTCCAC
CAGAGCTCCCTAATTCAGTATACAGGGAGATAGTGGTTCCACCGTGTCGCATTTTTTATCGTGAGGATGATAAGAGAGTT
CTCATTCTCTATGTCATGCGAGAGGAGCGACAGCTTCGGGCGTACATGCTTGGGAACAGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT214914 WP_000356397.1 NZ_CP082774:176094-176384 [Escherichia coli O16:H6]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT214914 NZ_CP110342:4087479-4087736 [Marinobacter sp. AN1]
ATGAAAGTTGAGCTGGTTACGAACCTCAAGCGCCAAGCCACAAAGATTCTGGCAGATCTGCACCTGACTAAAGAGCCGAT
ACTGATCACGGAGCACGGTCAGCCATCCGCATACCTTGTCGATGTGCAAGATTACGAGTTTATGCAGCGCCGACTTGAGC
TGCTTGAGGGGCTCTCACGAGGAGAGCGGGCTGTACTTGAGGGAAGAACGTACAGTCAAAGTGAGGCCATGGAAAAAATG
AGTAAATGGCTGAAGTAA

Structures

Toxin

• AlphaFold DB: S1P416