Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4236141..4236940 Replicon chromosome
Accession NZ_CP082773
Organism Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag K8B87_RS20325 Protein ID WP_000347251.1
Coordinates 4236476..4236940 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag K8B87_RS20320 Protein ID WP_001296435.1
Coordinates 4236141..4236476 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B87_RS20305 (4231926) 4231926..4232696 - 771 WP_087602804.1 2-dehydro-3-deoxyglucarate aldolase -
K8B87_RS20310 (4232712) 4232712..4234046 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
K8B87_RS20315 (4234421) 4234421..4235992 + 1572 WP_001273763.1 galactarate dehydratase -
K8B87_RS20320 (4236141) 4236141..4236476 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K8B87_RS20325 (4236476) 4236476..4236940 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K8B87_RS20330 (4236995) 4236995..4237804 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K8B87_RS20335 (4238053) 4238053..4239333 + 1281 WP_000681926.1 tagatose-bisphosphate aldolase subunit KbaZ -
K8B87_RS20340 (4239356) 4239356..4239829 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
K8B87_RS20345 (4239840) 4239840..4240619 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K8B87_RS20350 (4240609) 4240609..4241487 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
K8B87_RS20355 (4241505) 4241505..4241939 + 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T214913 WP_000347251.1 NZ_CP082773:4236476-4236940 [Escherichia coli O99:H6]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T214913 NZ_CP110342:4087073-4087336 [Marinobacter sp. AN1]
GTGACATGGAAGTTGGTTTACACCAAGCAAGCCCAGAAAGATGCAAAGAAGTTGGCTTCCAGCGGCCTTAAACCAAAAGC
TCAGGAACTTTTAGCGCTGATAGCGGAAGATCCATACCGTAAGCCGCCTCCGTTTGAGAAGCTCATTGGTGATCTTTCGG
GGGCCTATTCACGCCGCATTAATATTCAACATCGTCTGGTTTATCAGGTACTGGAAGACGAGCGGGTGGTGAAAGTTCTC
AGGCTCTGGAGCCACTACGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT214913 WP_001296435.1 NZ_CP082773:4236141-4236476 [Escherichia coli O99:H6]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT214913 NZ_CP110342:4087479-4087736 [Marinobacter sp. AN1]
ATGAAAGTTGAGCTGGTTACGAACCTCAAGCGCCAAGCCACAAAGATTCTGGCAGATCTGCACCTGACTAAAGAGCCGAT
ACTGATCACGGAGCACGGTCAGCCATCCGCATACCTTGTCGATGTGCAAGATTACGAGTTTATGCAGCGCCGACTTGAGC
TGCTTGAGGGGCTCTCACGAGGAGAGCGGGCTGTACTTGAGGGAAGAACGTACAGTCAAAGTGAGGCCATGGAAAAAATG
AGTAAATGGCTGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References