Detailed information of TA system TA214909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3677458..3678185
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: K8B87_RS17670
• Protein ID: WP_000547563.1
• Coordinates: 3677874..3678185 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B87_RS17665
• Protein ID: WP_000126304.1
• Coordinates: 3677458..3677877 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS17650 (3673621)	3673621..3675873	-	2253	WP_087602763.1	carbamoyltransferase HypF	-
K8B87_RS17655 (3676026)	3676026..3676553	-	528	WP_001078777.1	electron transport protein HydN	-
K8B87_RS17660 (3676938)	3676938..3677366	+	429	WP_000536064.1	hypothetical protein	-
K8B87_RS17665 (3677458)	3677458..3677877	-	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
K8B87_RS17670 (3677874)	3677874..3678185	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
K8B87_RS17675 (3678348)	3678348..3679103	-	756	WP_087602734.1	hypothetical protein	-
K8B87_RS17680 (3679146)	3679146..3679616	-	471	WP_000132954.1	hydrogenase maturation peptidase HycI	-
K8B87_RS17685 (3679609)	3679609..3680019	-	411	WP_001291913.1	formate hydrogenlyase assembly protein HycH	-
K8B87_RS17690 (3680016)	3680016..3680783	-	768	WP_087602735.1	formate hydrogenlyase subunit HycG	-
K8B87_RS17695 (3680783)	3680783..3681325	-	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
K8B87_RS17700 (3681335)	3681335..3683044	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T214909 WP_000547563.1 NZ_CP082773:c3678185-3677874 [Escherichia coli O99:H6]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T214909 NZ_CP110339:c4187518-4187096 [Brucella sp. JSBI001]
ATGATCCTTCTGGACACGAATGTCGTCTCGGAGGCGATGAAGCCGGAACAAGCTCCGGCCGTGCGCGACTGGCTGGACGA
ACAGGCGGCCGAAACGCTCTACCTTTCCAGTGTGACCGTGGCGGAACTCCTGTTCGGCATCGGCGCGATGCCTGACGGGC
GGCGCAAGCAGCGTCTCGCGACCACGCTCGACGGGCTTCTGCCGCTGTTCGAGGGCAGAATCCTTCCTTTCGACACGAAC
GCCGCCCGCCACTATGCCGACTTGGCCGTCGCGGCTCGCAAGGCCGGCAAGGGATTCCCCACACCTGACGGATATATCGC
CGCCATCGCCGCCGATCAGGGCTTTGCGGTCGCCACGCGTGACGCCAGCGCCTTCAACGCCGCCGGTGTTCCGGTCATCG
ACCCCTGGACAGCCGGACACTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT214909 WP_000126304.1 NZ_CP082773:c3677877-3677458 [Escherichia coli O99:H6]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT214909 NZ_CP110339:c4187769-4187515 [Brucella sp. JSBI001]
ATGCCCGCCGTCACGATCCGCAATCTGTCCGATGAGACACATCGTGCCCTCAAGGTGCGCGCGGCCCATCATGGCCGCAG
CACCGAGGCCGAGATGCGCGATATTCTGGAAGCAGCGGTTCGCCCCACGGACCGCCTTCGGATCGGCTCGGCCCTGTCGG
CATTGAGCCGCGAAGCCGGGCTCACCAACGCCGATGTCGAGGCGTTGGAAAAGGGCCGCGACCGGACACCCGCCACGCCC
ATGAGCTTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22