Detailed information of TA system TA214907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2969431..2969933
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: K8B87_RS14440
• Protein ID: WP_001560606.1
• Coordinates: 2969431..2969685 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: K8B87_RS14445
• Protein ID: WP_001259255.1
• Coordinates: 2969682..2969933 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS14410 (2964446)	2964446..2964673	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
K8B87_RS14415 (2964687)	2964687..2965745	-	1059	WP_042343276.1	thiosulfate utilization transporter TsuA/YeeE	-
K8B87_RS14420 (2965924)	2965924..2967282	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
K8B87_RS14425 (2967272)	2967272..2967334	-	63	WP_097433929.1	membrane protein YoeI	-
K8B87_RS14430 (2967549)	2967549..2968478	-	930	WP_023565113.1	LysR substrate-binding domain-containing protein	-
K8B87_RS14435 (2968524)	2968524..2969348	-	825	WP_000754737.1	SDR family oxidoreductase	-
K8B87_RS14440 (2969431)	2969431..2969685	-	255	WP_001560606.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
K8B87_RS14445 (2969682)	2969682..2969933	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
K8B87_RS14450 (2970216)	2970216..2970266	+	51	WP_001364200.1	his operon leader peptide	-
K8B87_RS14455 (2970412)	2970412..2971311	+	900	WP_087602615.1	ATP phosphoribosyltransferase	-
K8B87_RS14460 (2971317)	2971317..2972621	+	1305	WP_087602616.1	histidinol dehydrogenase	-
K8B87_RS14465 (2972618)	2972618..2973688	+	1071	WP_023565116.1	histidinol-phosphate transaminase	-
K8B87_RS14470 (2973688)	2973688..2974755	+	1068	WP_023565117.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10153.60 Da
• Isoelectric Point: 8.8560

>T214907 WP_001560606.1 NZ_CP082773:c2969685-2969431 [Escherichia coli O99:H6]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEVHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T214907 NZ_CP110339:c2207875-2207474 [Brucella sp. JSBI001]
ATGCTGAAGTACATGCTGGATACAAATATATGCATTTTCACGATCAAGAACCGGCCGCAGCAAGTGCGCGAGGCGTTCAA
CCGCTTCCACGATCAATTGTGCATCAGCTCAGTCAGTTTAATGGAGCTGATTTACGGGGCGGAGAAGTCTGCCCATCCTG
AAAAGAACCTTGCAGTCGTGGAAGGGTTCGCAGCTCGCCTTGAGGTGTTATCGTATGATGAACCGGCAGCAAACCATACG
GGCCAATTACGTGCTGAATTGGCCCGCAGTGGAACTCCAATCGGTCCATACGATCAATTGATTGCTGGCCATGCTCGCTC
GCGTGGCCTGATAATCGTTACAAACAATCGCACGGAATTTGATCGTGTTCCTGGCTTGCGAGTAGAAGACTGGACTGATT
AA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT214907 WP_001259255.1 NZ_CP082773:c2969933-2969682 [Escherichia coli O99:H6]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT214907 NZ_CP110339:c2208105-2207875 [Brucella sp. JSBI001]
ATGGAGCAGGGAGCAGTATTCCAGAGCAACCGGAGCCAAGCTATCCGCTTGCCAAAAGCAGTAGCATTGCCTGCTGATGT
GAAACGCGTTGATATTGTTGCTGTGGGGCGCACTCGAATCATAGCACCCGCTGGTGAGGTCTGGGATAGCTGGTTTGATA
GCTTAGAGGCGACTTCCGACTTCATGAATGAGCGTGATCAGCCTGCTCCACAGGAACGCGAGGCGTTCTAA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0