214897

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2435299..2435861 Replicon chromosome
Accession NZ_CP082773
Organism Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag K8B87_RS11725 Protein ID WP_000605675.1
Coordinates 2435583..2435861 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag K8B87_RS11720 Protein ID WP_000781370.1
Coordinates 2435299..2435583 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B87_RS11705 (2430480) 2430480..2433527 + 3048 WP_140395589.1 formate dehydrogenase-N subunit alpha -
K8B87_RS11710 (2433540) 2433540..2434424 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
K8B87_RS11715 (2434417) 2434417..2435070 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
K8B87_RS11720 (2435299) 2435299..2435583 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
K8B87_RS11725 (2435583) 2435583..2435861 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B87_RS11730 (2436047) 2436047..2437057 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
K8B87_RS11735 (2437191) 2437191..2438888 - 1698 WP_000433462.1 malate dehydrogenase -
K8B87_RS11740 (2439045) 2439045..2439182 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
K8B87_RS11745 (2439284) 2439284..2439499 - 216 WP_000495766.1 biofilm-dependent modulation protein -
K8B87_RS11750 (2439844) 2439844..2440275 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T214897 WP_000605675.1 NZ_CP082773:c2435861-2435583 [Escherichia coli O99:H6]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T214897 NZ_CP110314:c48522-48106 [Xanthomonas phaseoli pv. phaseoli]
ATGATCGTTCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGCCGCACCCATCCGTGCGGACCTGGTTGAACGA
TCAAGCCGCTGAAACACTGTACCTGTCCAGCGTGACGCTGGCCGAACTGCTATTTGGCATCGCTGCGCTTCCTGCCGGTA
AGCGCAAGGACATGCTGGAACAGGCCCTTGATGGCCTCATGGGCCTGTTTAGGGATCGGGTGTTGCCATTCGACATCGAT
GCAGCGCGGCGTTATGCCGAATTGGCCGTGACGGCAAAGATCGGAGGGCGAGGATTTCCGACACCCGACGGCTACATCGC
AGCGATTGCTGCCTCGCGGGGTTTCATTGTGGCGTCACGCGACACGGCCCCTTACGAAGCGGCCGGCGTAACCGTCATCA
ATCCGTGGGAAGGGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT214897 WP_000781370.1 NZ_CP082773:c2435583-2435299 [Escherichia coli O99:H6]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT214897 NZ_CP110314:c48773-48519 [Xanthomonas phaseoli pv. phaseoli]
ATGGCAATGCTGACAGTACGTAATCTGCCGGATGATGTGCACCGCGCATTGCGCATGCGGGCCGCTCAAAATGGGCACAG
CACCGAGGCCGAGGTCCGCGAGATTCTGGCGACCGCAGTCAAGCCAGAGACGCGCGTTCGCCTAGGCGAAGCCTTGGCGG
CTTTGGGTCGCAAGATTGGCCTGACGAATGAGGACTTCGAGGTCTTCGATCGGGTAAGAGACAAGACGCCGGCCGTCCCG
CCGAGGCTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References