Detailed information of TA system TA214889

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 764703..765112
• Replicon: chromosome
• Accession: NZ_CP082773
• Organism: Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A3L4RJH9
• Locus tag: K8B87_RS03600
• Protein ID: WP_001357525.1
• Coordinates: 764703..765041 (-)
• Length: 113 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	765036..765112 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B87_RS03575 (760361)	760361..761512	-	1152	WP_001298064.1	double-cubane-cluster-containing anaerobic reductase	-
K8B87_RS03580 (761628)	761628..762908	-	1281	WP_012601894.1	DUF445 domain-containing protein	-
K8B87_RS03585 (763093)	763093..764013	+	921	WP_071342725.1	Rpn family recombination-promoting nuclease/putative transposase	-
K8B87_RS03590 (764005)	764005..764319	-	315	Protein_685	winged helix-turn-helix domain-containing protein	-
K8B87_RS03595 (764497)	764497..764661	+	165	WP_001357524.1	DUF1127 domain-containing protein	-
K8B87_RS03600 (764703)	764703..765041	-	339	WP_001357525.1	endoribonuclease SymE	Toxin
- (765036)	765036..765112	+	77	NuclAT_7	-	Antitoxin
- (765036)	765036..765112	+	77	NuclAT_7	-	Antitoxin
- (765036)	765036..765112	+	77	NuclAT_7	-	Antitoxin
- (765036)	765036..765112	+	77	NuclAT_7	-	Antitoxin
- (765036)	765036..765112	+	77	NuclAT_8	-	Antitoxin
- (765036)	765036..765112	+	77	NuclAT_8	-	Antitoxin
- (765036)	765036..765112	+	77	NuclAT_8	-	Antitoxin
- (765036)	765036..765112	+	77	NuclAT_8	-	Antitoxin
K8B87_RS03605 (765256)	765256..765465	-	210	Protein_688	DUF3387 domain-containing protein	-
K8B87_RS03610 (765694)	765694..767544	-	1851	WP_087603013.1	nuclease-related domain-containing DEAD/DEAH box helicase	-
K8B87_RS03615 (767875)	767875..768831	-	957	WP_001314406.1	GTPase	-
K8B87_RS03620 (768842)	768842..769045	-	204	WP_000467859.1	YbdD/YjiX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	764703..776526	11823

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12314.03 Da
• Isoelectric Point: 7.8234

>T214889 WP_001357525.1 NZ_CP082773:c765041-764703 [Escherichia coli O99:H6]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFTTGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQRQVQDFIGVISSKTPR

Nucleotide

• Length: 339 bp

>T214889 NZ_CP110309:c4458335-4458201 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT214889 NZ_CP082773:765036-765112 [Escherichia coli O99:H6]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A3L4RJH9

Antitoxin