Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 173911..174513 Replicon chromosome
Accession NZ_CP082773
Organism Escherichia coli O99:H6 strain CU42ET-1

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag K8B87_RS00840 Protein ID WP_000897302.1
Coordinates 173911..174222 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K8B87_RS00845 Protein ID WP_000356397.1
Coordinates 174223..174513 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B87_RS00815 (169825) 169825..170424 + 600 WP_001314324.1 glucose-1-phosphatase -
K8B87_RS00820 (170418) 170418..171290 + 873 WP_000920762.1 virulence factor BrkB family protein -
K8B87_RS00825 (171287) 171287..171724 + 438 WP_001553787.1 D-aminoacyl-tRNA deacylase -
K8B87_RS00830 (171769) 171769..172710 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
K8B87_RS00835 (172774) 172774..173682 - 909 WP_087602929.1 alpha/beta hydrolase -
K8B87_RS00840 (173911) 173911..174222 + 312 WP_000897302.1 hypothetical protein Toxin
K8B87_RS00845 (174223) 174223..174513 + 291 WP_000356397.1 NadS family protein Antitoxin
K8B87_RS00850 (174872) 174872..175150 + 279 WP_234257669.1 hypothetical protein -
K8B87_RS00855 (175546) 175546..175764 + 219 WP_001314326.1 CopG family transcriptional regulator -
K8B87_RS00860 (175986) 175986..176915 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
K8B87_RS00865 (176912) 176912..177547 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
K8B87_RS00870 (177544) 177544..178446 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T214885 WP_000897302.1 NZ_CP082773:173911-174222 [Escherichia coli O99:H6]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T214885 NZ_CP110306:c4425200-4425048 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGTCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAGTGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT214885 WP_000356397.1 NZ_CP082773:174223-174513 [Escherichia coli O99:H6]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT214885 NZ_CP110306:c4424973-4424839 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References