214884

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4476058..4476857 Replicon chromosome
Accession NZ_CP082772
Organism Escherichia coli O2:H1 strain HM670

Toxin (Protein)


Gene name yhaV Uniprot ID Q8FDB4
Locus tag K8B93_RS21555 Protein ID WP_000347252.1
Coordinates 4476393..4476857 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag K8B93_RS21550 Protein ID WP_001296435.1
Coordinates 4476058..4476393 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B93_RS21535 (4471843) 4471843..4472613 - 771 WP_001058223.1 2-dehydro-3-deoxyglucarate aldolase -
K8B93_RS21540 (4472629) 4472629..4473963 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
K8B93_RS21545 (4474338) 4474338..4475909 + 1572 WP_001273940.1 galactarate dehydratase -
K8B93_RS21550 (4476058) 4476058..4476393 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K8B93_RS21555 (4476393) 4476393..4476857 + 465 WP_000347252.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K8B93_RS21560 (4476912) 4476912..4477721 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K8B93_RS21565 (4477970) 4477970..4479250 + 1281 WP_000681926.1 tagatose-bisphosphate aldolase subunit KbaZ -
K8B93_RS21570 (4479273) 4479273..4479746 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K8B93_RS21575 (4479757) 4479757..4480536 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K8B93_RS21580 (4480526) 4480526..4481404 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
K8B93_RS21585 (4481422) 4481422..4481856 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17750.11 Da        Isoelectric Point: 9.4947

>T214884 WP_000347252.1 NZ_CP082772:4476393-4476857 [Escherichia coli O2:H1]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Download         Length: 465 bp

>T214884 NZ_CP110306:c4424973-4424839 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT214884 WP_001296435.1 NZ_CP082772:4476058-4476393 [Escherichia coli O2:H1]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT214884 NZ_CP110306:c4425200-4425048 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGTCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAGTGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8FDB4

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References