Detailed information of TA system TA214880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3895944..3896671
• Replicon: chromosome
• Accession: NZ_CP082772
• Organism: Escherichia coli O2:H1 strain HM670

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: K8B93_RS18840
• Protein ID: WP_000547555.1
• Coordinates: 3896360..3896671 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0H2V9Z2
• Locus tag: K8B93_RS18835
• Protein ID: WP_000126293.1
• Coordinates: 3895944..3896363 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B93_RS18820 (3892107)	3892107..3894359	-	2253	WP_001107659.1	carbamoyltransferase HypF	-
K8B93_RS18825 (3894512)	3894512..3895039	-	528	WP_001078777.1	electron transport protein HydN	-
K8B93_RS18830 (3895424)	3895424..3895852	+	429	WP_000536064.1	hypothetical protein	-
K8B93_RS18835 (3895944)	3895944..3896363	-	420	WP_000126293.1	helix-turn-helix domain-containing protein	Antitoxin
K8B93_RS18840 (3896360)	3896360..3896671	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
K8B93_RS18845 (3896834)	3896834..3897589	-	756	WP_001305268.1	hypothetical protein	-
K8B93_RS18850 (3897632)	3897632..3898102	-	471	WP_000132956.1	hydrogenase maturation peptidase HycI	-
K8B93_RS18855 (3898095)	3898095..3898505	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
K8B93_RS18860 (3898502)	3898502..3899269	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
K8B93_RS18865 (3899269)	3899269..3899811	-	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
K8B93_RS18870 (3899821)	3899821..3901532	-	1712	Protein_3668	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T214880 WP_000547555.1 NZ_CP082772:c3896671-3896360 [Escherichia coli O2:H1]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T214880 NZ_CP082772:c3896671-3896360 [Escherichia coli O2:H1]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15426.46 Da
• Isoelectric Point: 4.4596

>AT214880 WP_000126293.1 NZ_CP082772:c3896363-3895944 [Escherichia coli O2:H1]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT214880 NZ_CP082772:c3896363-3895944 [Escherichia coli O2:H1]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2

Antitoxin

• AlphaFold DB: A0A0H2V9Z2