Detailed information of TA system TA214874

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hokW-rdlD/-
• Location: 3032168..3032393
• Replicon: chromosome
• Accession: NZ_CP082772
• Organism: Escherichia coli O2:H1 strain HM670

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: K8B93_RS15035
• Protein ID: WP_000813254.1
• Coordinates: 3032168..3032323 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	3032335..3032393 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B93_RS14980	3027248..3027562	-	315	WP_000193273.1	YdfR family protein	-
K8B93_RS14985	3027567..3027782	-	216	WP_000839572.1	class II holin family protein	-
K8B93_RS15010	3028507..3029220	-	714	WP_000342820.1	hypothetical protein	-
K8B93_RS15015	3029466..3030287	-	822	WP_001235237.1	antitermination protein	-
K8B93_RS15020	3030284..3030658	-	375	WP_001217427.1	RusA family crossover junction endodeoxyribonuclease	-
K8B93_RS15025	3030671..3031720	-	1050	WP_001265292.1	DUF968 domain-containing protein	-
K8B93_RS15030	3031722..3032000	-	279	WP_024193993.1	hypothetical protein	-
K8B93_RS15035	3032168..3032323	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3032335..3032393	+	59	-	-	Antitoxin
K8B93_RS15040	3032561..3033226	-	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
K8B93_RS15045	3033401..3033826	-	426	WP_001151152.1	DUF977 family protein	-
K8B93_RS15050	3033867..3034937	-	1071	WP_001262355.1	hypothetical protein	-
K8B93_RS15055	3035009..3035434	-	426	WP_000693918.1	toxin YdaT family protein	-
K8B93_RS15060	3035418..3035690	-	273	WP_000887447.1	YdaS family helix-turn-helix protein	-
K8B93_RS15065	3035800..3036201	+	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
K8B93_RS15070	3036229..3036420	+	192	WP_000100899.1	hypothetical protein	-
K8B93_RS15075	3036420..3036707	+	288	WP_000936799.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K8B93_RS15080	3036979..3037131	+	153	WP_000379609.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtS / ybtX / ybtQ / ybtP	2999928..3052345	52417

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T214874 WP_000813254.1 NZ_CP082772:c3032323-3032168 [Escherichia coli O2:H1]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T214874 NZ_CP110301:65602-65880 [Xanthomonas citri pv. fuscans]
GTGCCGGCATTGGCTGATCTGGACGCAATCGCCGACTACATCGCAATCGATAACGCACCCGCTGCAGCGGCGCTGGTGAA
ACGGGTGTTCGCGCACGTTGAGCAATTGATTGAACACCCGGACAGCGGAAGCCGGCCACAGGAACTCAAGCGCTCGCGCT
ATCGCCAGATTGTCGAACCACCTTGCCGCGTGTTCTACCGCGTGGATGGGCAGCGCATCGTGGTGGTGCACGTAATGCGG
TCGGAGCGCGCGCTTCGCGGGAACCGTCTTTCACGCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT214874 NZ_CP082772:3032335-3032393 [Escherichia coli O2:H1]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin