214864

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2484213..2484775 Replicon chromosome
Accession NZ_CP082772
Organism Escherichia coli O2:H1 strain HM670

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag K8B93_RS12070 Protein ID WP_000605675.1
Coordinates 2484497..2484775 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag K8B93_RS12065 Protein ID WP_000781370.1
Coordinates 2484213..2484497 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B93_RS12050 (2479394) 2479394..2482441 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
K8B93_RS12055 (2482454) 2482454..2483338 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
K8B93_RS12060 (2483331) 2483331..2483984 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
K8B93_RS12065 (2484213) 2484213..2484497 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
K8B93_RS12070 (2484497) 2484497..2484775 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B93_RS12075 (2484961) 2484961..2485971 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
K8B93_RS12080 (2486105) 2486105..2487802 - 1698 WP_000433460.1 malate dehydrogenase -
K8B93_RS12085 (2487959) 2487959..2488096 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
K8B93_RS12090 (2488198) 2488198..2488413 - 216 WP_000495766.1 biofilm-dependent modulation protein -
K8B93_RS12095 (2488758) 2488758..2489189 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T214864 WP_000605675.1 NZ_CP082772:c2484775-2484497 [Escherichia coli O2:H1]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T214864 NZ_CP110296:65597-65875 [Xanthomonas citri pv. fuscans]
GTGCCGGCATTGGCTGATCTGGACGCAATCGCCGACTACATCGCAATCGATAACGCACCCGCTGCAGCGGCGCTGGTGAA
ACGGGTGTTCGCGCACGTTGAGCAATTGATTGAACACCCGGACAGCGGAAGCCGGCCACAGGAACTCAAGCGCTCGCGCT
ATCGCCAGATTGTCGAACCACCTTGCCGCGTGTTCTACCGCGTGGATGGGCAGCGCATCGTGGTGGTGCACGTAATGCGG
TCGGAGCGCGCGCTTCGCGGGAACCGTCTTTCACGCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT214864 WP_000781370.1 NZ_CP082772:c2484497-2484213 [Escherichia coli O2:H1]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT214864 NZ_CP110296:65331-65588 [Xanthomonas citri pv. fuscans]
ATGCGCACCGAACTTGTCACCACGCTCAAGCGTCAGGCGACCGAACTCCTTGCAGCTGCAGAGCGCGACAAGGAGCCAAT
CCTGATCACACAGCACGGTTTACCCAGTGCCTATCTGGTGGATGTTGCCAGCTATGAGCGCATGCAGCAGCGCATTGCGC
TCCTCGAAGGAATCGCCCGGGGAGAAATGGGAGTCGCGGACGGCCGTACGCTGAGCCATGAACAGGCACAGCAACGCATG
GCGCGATGGCTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References