Detailed information of TA system TA214857

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 828549..828961
• Replicon: chromosome
• Accession: NZ_CP082772
• Organism: Escherichia coli O2:H1 strain HM670

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: K8B93_RS03855
• Protein ID: WP_000132630.1
• Coordinates: 828549..828890 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	828885..828961 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B93_RS03830 (823618)	823618..824898	-	1281	WP_001304526.1	DUF445 domain-containing protein	-
K8B93_RS03835 (825083)	825083..826003	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
K8B93_RS03840 (826231)	826231..826311	+	81	WP_020233658.1	hypothetical protein	-
K8B93_RS03845 (826409)	826409..827291	+	883	Protein_736	DUF262 domain-containing protein	-
K8B93_RS03850 (827339)	827339..828502	+	1164	WP_072202028.1	DUF1524 domain-containing protein	-
K8B93_RS03855 (828549)	828549..828890	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
- (828885)	828885..828961	+	77	NuclAT_7	-	Antitoxin
- (828885)	828885..828961	+	77	NuclAT_7	-	Antitoxin
- (828885)	828885..828961	+	77	NuclAT_7	-	Antitoxin
- (828885)	828885..828961	+	77	NuclAT_7	-	Antitoxin
- (828885)	828885..828961	+	77	NuclAT_8	-	Antitoxin
- (828885)	828885..828961	+	77	NuclAT_8	-	Antitoxin
- (828885)	828885..828961	+	77	NuclAT_8	-	Antitoxin
- (828885)	828885..828961	+	77	NuclAT_8	-	Antitoxin
K8B93_RS03860 (829111)	829111..830880	-	1770	WP_000110076.1	restriction endonuclease subunit S	-
K8B93_RS03865 (830880)	830880..832349	-	1470	WP_001315223.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T214857 WP_000132630.1 NZ_CP082772:c828890-828549 [Escherichia coli O2:H1]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T214857 NZ_CP110289:2730004-2730144 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT214857 NZ_CP082772:828885-828961 [Escherichia coli O2:H1]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin