Detailed information of TA system TA214851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4125643..4126442
• Replicon: chromosome
• Accession: NZ_CP082771
• Organism: Escherichia coli LF82

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: K8B90_RS19925
• Protein ID: WP_000347252.1
• Coordinates: 4125978..4126442 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: K8B90_RS19920
• Protein ID: WP_001296435.1
• Coordinates: 4125643..4125978 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B90_RS19905 (4121428)	4121428..4122198	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
K8B90_RS19910 (4122214)	4122214..4123548	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
K8B90_RS19915 (4123923)	4123923..4125494	+	1572	WP_001273939.1	galactarate dehydratase	-
K8B90_RS19920 (4125643)	4125643..4125978	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
K8B90_RS19925 (4125978)	4125978..4126442	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
K8B90_RS19930 (4126497)	4126497..4127306	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
K8B90_RS19935 (4127555)	4127555..4128835	+	1281	WP_000681923.1	tagatose-bisphosphate aldolase subunit KbaZ	-
K8B90_RS19940 (4128858)	4128858..4129331	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
K8B90_RS19945 (4129342)	4129342..4130121	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
K8B90_RS19950 (4130111)	4130111..4130989	+	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
K8B90_RS19955 (4131007)	4131007..4131441	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T214851 WP_000347252.1 NZ_CP082771:4125978-4126442 [Escherichia coli LF82]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T214851 NZ_CP110289:414395-414703 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT214851 WP_001296435.1 NZ_CP082771:4125643-4125978 [Escherichia coli LF82]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT214851 NZ_CP110289:414121-414393 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5