Detailed information of TA system TA214848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2911528..2912030
• Replicon: chromosome
• Accession: NZ_CP082771
• Organism: Escherichia coli LF82

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: K8B90_RS14290
• Protein ID: WP_000767819.1
• Coordinates: 2911528..2911782 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: K8B90_RS14295
• Protein ID: WP_001259253.1
• Coordinates: 2911779..2912030 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B90_RS14270 (2908021)	2908021..2909379	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
K8B90_RS14275 (2909369)	2909369..2909431	-	63	WP_010723108.1	membrane protein YoeI	-
K8B90_RS14280 (2909646)	2909646..2910575	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
K8B90_RS14285 (2910621)	2910621..2911445	-	825	WP_000754733.1	SDR family oxidoreductase	-
K8B90_RS14290 (2911528)	2911528..2911782	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
K8B90_RS14295 (2911779)	2911779..2912030	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
K8B90_RS14300 (2912313)	2912313..2912363	+	51	WP_001368802.1	his operon leader peptide	-
K8B90_RS14305 (2912509)	2912509..2913408	+	900	WP_000131791.1	ATP phosphoribosyltransferase	-
K8B90_RS14310 (2913414)	2913414..2914718	+	1305	WP_000009596.1	histidinol dehydrogenase	-
K8B90_RS14315 (2914715)	2914715..2915785	+	1071	WP_000108931.1	histidinol-phosphate transaminase	-
K8B90_RS14320 (2915785)	2915785..2916852	+	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T214848 WP_000767819.1 NZ_CP082771:c2911782-2911528 [Escherichia coli LF82]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T214848 NZ_CP110285:2314660-2315397 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT214848 WP_001259253.1 NZ_CP082771:c2912030-2911779 [Escherichia coli LF82]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT214848 NZ_CP110285:2315510-2315635 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3