Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2318773..2319335 Replicon chromosome
Accession NZ_CP082771
Organism Escherichia coli LF82

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag K8B90_RS11255 Protein ID WP_000605675.1
Coordinates 2319057..2319335 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag K8B90_RS11250 Protein ID WP_000781370.1
Coordinates 2318773..2319057 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B90_RS11235 (2313954) 2313954..2317001 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
K8B90_RS11240 (2317014) 2317014..2317898 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
K8B90_RS11245 (2317891) 2317891..2318544 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
K8B90_RS11250 (2318773) 2318773..2319057 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
K8B90_RS11255 (2319057) 2319057..2319335 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8B90_RS11260 (2319521) 2319521..2320531 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
K8B90_RS11265 (2320665) 2320665..2322362 - 1698 WP_000433464.1 malate dehydrogenase -
K8B90_RS11270 (2322519) 2322519..2322656 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
K8B90_RS11275 (2322758) 2322758..2322973 - 216 WP_000495766.1 biofilm-dependent modulation protein -
K8B90_RS11280 (2323318) 2323318..2323749 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T214837 WP_000605675.1 NZ_CP082771:c2319335-2319057 [Escherichia coli LF82]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T214837 NZ_CP110266:40128-40439 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT214837 WP_000781370.1 NZ_CP082771:c2319057-2318773 [Escherichia coli LF82]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT214837 NZ_CP110266:40436-40855 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References