Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1089746..1090440 Replicon chromosome
Accession NZ_CP082771
Organism Escherichia coli LF82

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag K8B90_RS05120 Protein ID WP_001263501.1
Coordinates 1090042..1090440 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag K8B90_RS05115 Protein ID WP_000554755.1
Coordinates 1089746..1090039 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B90_RS05090 (1085184) 1085184..1085681 + 498 WP_000006238.1 REP-associated tyrosine transposase RayT -
K8B90_RS05095 (1085676) 1085676..1086020 - 345 WP_137486338.1 hypothetical protein -
K8B90_RS05100 (1086099) 1086099..1087811 - 1713 Protein_978 flagellar biosynthesis protein FlhA -
K8B90_RS05105 (1087783) 1087783..1088568 + 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
K8B90_RS05110 (1088639) 1088639..1089694 + 1056 WP_001226167.1 DNA polymerase IV -
K8B90_RS05115 (1089746) 1089746..1090039 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
K8B90_RS05120 (1090042) 1090042..1090440 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
K8B90_RS05125 (1090450) 1090450..1090902 + 453 WP_001059867.1 GNAT family N-acetyltransferase -
K8B90_RS05130 (1091219) 1091219..1092358 + 1140 WP_000521551.1 RNA ligase RtcB family protein -
K8B90_RS05135 (1092355) 1092355..1092969 + 615 WP_000602135.1 peptide chain release factor H -
K8B90_RS05140 (1093026) 1093026..1094483 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
K8B90_RS05145 (1094744) 1094744..1095202 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T214835 WP_001263501.1 NZ_CP082771:1090042-1090440 [Escherichia coli LF82]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T214835 NZ_CP110265:c5123280-5122897 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT214835 WP_000554755.1 NZ_CP082771:1089746-1090039 [Escherichia coli LF82]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT214835 NZ_CP110265:c5123522-5123280 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References