Detailed information of TA system TA214828

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 735176..735588
• Replicon: chromosome
• Accession: NZ_CP082771
• Organism: Escherichia coli LF82

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: K8B90_RS03460
• Protein ID: WP_000132614.1
• Coordinates: 735176..735517 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	735512..735588 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B90_RS03435 (730245)	730245..731525	-	1281	WP_014640274.1	DUF445 domain-containing protein	-
K8B90_RS03440 (731710)	731710..732630	+	921	WP_000181192.1	Rpn family recombination-promoting nuclease/putative transposase	-
K8B90_RS03445 (732858)	732858..732938	+	81	WP_020233658.1	hypothetical protein	-
K8B90_RS03450 (733036)	733036..733918	+	883	Protein_657	DUF262 domain-containing protein	-
K8B90_RS03455 (733966)	733966..735129	+	1164	WP_072002093.1	DUF1524 domain-containing protein	-
K8B90_RS03460 (735176)	735176..735517	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
- (735512)	735512..735588	+	77	NuclAT_6	-	Antitoxin
- (735512)	735512..735588	+	77	NuclAT_6	-	Antitoxin
- (735512)	735512..735588	+	77	NuclAT_6	-	Antitoxin
- (735512)	735512..735588	+	77	NuclAT_6	-	Antitoxin
- (735512)	735512..735588	+	77	NuclAT_7	-	Antitoxin
- (735512)	735512..735588	+	77	NuclAT_7	-	Antitoxin
- (735512)	735512..735588	+	77	NuclAT_7	-	Antitoxin
- (735512)	735512..735588	+	77	NuclAT_7	-	Antitoxin
K8B90_RS03465 (735738)	735738..737444	-	1707	WP_001100193.1	restriction endonuclease subunit S	-
K8B90_RS03470 (737444)	737444..738913	-	1470	WP_001540814.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T214828 WP_000132614.1 NZ_CP082771:c735517-735176 [Escherichia coli LF82]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T214828 NZ_CP110265:1866753-1866855 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 77 bp

>AT214828 NZ_CP082771:735512-735588 [Escherichia coli LF82]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin