Detailed information of TA system TA214826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 174878..175480
• Replicon: chromosome
• Accession: NZ_CP082771
• Organism: Escherichia coli LF82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U9FTF1
• Locus tag: K8B90_RS00850
• Protein ID: WP_000897301.1
• Coordinates: 174878..175189 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8B90_RS00855
• Protein ID: WP_000356397.1
• Coordinates: 175190..175480 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B90_RS00825 (170793)	170793..171392	+	600	WP_001314324.1	glucose-1-phosphatase	-
K8B90_RS00830 (171386)	171386..172258	+	873	WP_000920762.1	virulence factor BrkB family protein	-
K8B90_RS00835 (172255)	172255..172692	+	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
K8B90_RS00840 (172737)	172737..173678	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
K8B90_RS00845 (173742)	173742..174650	-	909	WP_001331553.1	alpha/beta hydrolase	-
K8B90_RS00850 (174878)	174878..175189	+	312	WP_000897301.1	hypothetical protein	Toxin
K8B90_RS00855 (175190)	175190..175480	+	291	WP_000356397.1	NadS family protein	Antitoxin
K8B90_RS00860 (175839)	175839..176117	+	279	WP_001296612.1	hypothetical protein	-
K8B90_RS00865 (176513)	176513..176731	+	219	WP_001314326.1	CopG family transcriptional regulator	-
K8B90_RS00870 (176947)	176947..177876	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
K8B90_RS00875 (177873)	177873..178508	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
K8B90_RS00880 (178505)	178505..179407	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12150.14 Da
• Isoelectric Point: 8.8941

>T214826 WP_000897301.1 NZ_CP082771:174878-175189 [Escherichia coli LF82]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKCAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T214826 NZ_CP110265:729557-730042 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT214826 WP_000356397.1 NZ_CP082771:175190-175480 [Escherichia coli LF82]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT214826 NZ_CP110265:729267-729560 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A7U9FTF1