Detailed information of TA system TA214816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 40344..41071
• Replicon: plasmid pKP12-vir
• Accession: NZ_CP082766
• Organism: Klebsiella pneumoniae strain 12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: FTO26_RS27210
• Protein ID: WP_011251285.1
• Coordinates: 40344..40655 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FTO26_RS27215
• Protein ID: WP_011251286.1
• Coordinates: 40652..41071 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTO26_RS29770	35627..35956	-	330	WP_228751292.1	hypothetical protein	-
FTO26_RS27190 (FTO26_027190)	35955..36935	+	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
FTO26_RS27195 (FTO26_027195)	36993..37484	+	492	WP_147041491.1	DUF1173 family protein	-
FTO26_RS27205 (FTO26_027205)	39702..40139	+	438	Protein_42	DDE-type integrase/transposase/recombinase	-
FTO26_RS27210 (FTO26_027210)	40344..40655	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
FTO26_RS27215 (FTO26_027215)	40652..41071	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
FTO26_RS27220 (FTO26_027220)	41218..42186	+	969	WP_086642568.1	IS5 family transposase	-
FTO26_RS27225 (FTO26_027225)	42258..42623	-	366	WP_048333448.1	hypothetical protein	-
FTO26_RS27230 (FTO26_027230)	42637..43425	-	789	WP_040217257.1	hypothetical protein	-
FTO26_RS27235 (FTO26_027235)	43446..44066	-	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
FTO26_RS27240 (FTO26_027240)	44485..45120	+	636	WP_223171879.1	hypothetical protein	-
FTO26_RS27245 (FTO26_027245)	45433..45903	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..199279	199279
	-	inside	IScluster/Tn	-	-	22688..48911	26223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T214816 WP_011251285.1 NZ_CP082766:40344-40655 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T214816 NZ_CP110260:4081836-4082147 [Escherichia albertii]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT214816 WP_011251286.1 NZ_CP082766:40652-41071 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT214816 NZ_CP110260:4082144-4082563 [Escherichia albertii]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1