Detailed information of TA system TA214795

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132675..132928
• Replicon: plasmid pKP36-KPC
• Accession: NZ_CP082761
• Organism: Klebsiella pneumoniae strain 36

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FTO14_RS28940
• Protein ID: WP_001312851.1
• Coordinates: 132779..132928 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	132675..132734 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTO14_RS28900 (128057)	128057..128401	-	345	Protein_162	IS6-like element IS26 family transposase	-
FTO14_RS28905 (128453)	128453..129157	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FTO14_RS28910 (129182)	129182..129382	+	201	WP_072354025.1	hypothetical protein	-
FTO14_RS28915 (129402)	129402..130148	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
FTO14_RS28920 (130203)	130203..130763	+	561	WP_000139328.1	fertility inhibition protein FinO	-
FTO14_RS28925 (130895)	130895..131095	+	201	WP_015059022.1	hypothetical protein	-
FTO14_RS28930 (131481)	131481..132080	+	600	WP_032083981.1	PIN domain-containing protein	-
FTO14_RS28935 (132142)	132142..132474	+	333	WP_152916585.1	hypothetical protein	-
- (132675)	132675..132734	-	60	NuclAT_1	-	Antitoxin
- (132675)	132675..132734	-	60	NuclAT_1	-	Antitoxin
- (132675)	132675..132734	-	60	NuclAT_1	-	Antitoxin
- (132675)	132675..132734	-	60	NuclAT_1	-	Antitoxin
FTO14_RS28940 (132779)	132779..132928	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
FTO14_RS28945 (133212)	133212..133460	+	249	WP_000083837.1	replication regulatory protein RepA	-
FTO14_RS29830 (133575)	133575..133759	+	185	Protein_172	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133771	133771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T214795 WP_001312851.1 NZ_CP082761:132779-132928 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T214795 NZ_CP110258:915467-915871 [Escherichia albertii]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTCTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGCTATTTTACTCATGCCCTGGCCGCTAAGTTATACCCCGTTATGGATGGTGTTGCTTTCGCTGGTGGTGTTTGATTGCG
TCCGCAGTCAGCGGCGCATTAATGCTCGTCAGGGGGAGATTCGCCTGTTGATGGATGGGCGTTTACGCTGGCAGGGGCAG
GAGTGGAGCATCGTCAGAGCGCCGTGGATGATTAAAACTGGCATGATGTTGTGTTTACTCTCTGATAGCGGCAAACGTCA
GCACCTGTGGCTGGCAGCCGACAGTATGGATGAAGCTGAATGGCGGGATTTGCGGCGAATTTTGTTACAACAAGAGATAC
AGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT214795 NZ_CP082761:c132734-132675 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin