Detailed information of TA system TA214720

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41196..41465
• Replicon: plasmid pVCSW7
• Accession: NZ_CP082358
• Organism: Escherichia coli strain VCSW7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K7X31_RS22585
• Protein ID: WP_001372321.1
• Coordinates: 41340..41465 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41196..41261 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X31_RS22995	36240..36491	+	252	WP_071828952.1	hypothetical protein	-
K7X31_RS23000	36732..36938	+	207	WP_000547968.1	hypothetical protein	-
K7X31_RS22555	36964..37503	+	540	WP_105633039.1	single-stranded DNA-binding protein	-
K7X31_RS22560	37565..37798	+	234	WP_105633040.1	DUF905 family protein	-
K7X31_RS22565	37864..39822	+	1959	WP_223025133.1	ParB/RepB/Spo0J family partition protein	-
K7X31_RS22570	39877..40311	+	435	WP_105633042.1	conjugation system SOS inhibitor PsiB	-
K7X31_RS22575	40308..41070	+	763	Protein_45	plasmid SOS inhibition protein A	-
K7X31_RS22580	41039..41227	-	189	WP_001299721.1	hypothetical protein	-
-	41039..41263	+	225	NuclAT_0	-	-
-	41039..41263	+	225	NuclAT_0	-	-
-	41039..41263	+	225	NuclAT_0	-	-
-	41039..41263	+	225	NuclAT_0	-	-
-	41196..41261	-	66	-	-	Antitoxin
K7X31_RS23005	41249..41398	+	150	Protein_47	plasmid maintenance protein Mok	-
K7X31_RS22585	41340..41465	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K7X31_RS23010	41686..41916	+	231	WP_001426396.1	hypothetical protein	-
K7X31_RS23015	41914..42087	-	174	Protein_50	hypothetical protein	-
K7X31_RS23020	42157..42363	+	207	WP_000547968.1	hypothetical protein	-
K7X31_RS22590	42388..42675	+	288	WP_000107535.1	hypothetical protein	-
K7X31_RS22595	42759..43955	-	1197	WP_001358663.1	IS110-like element ISEc20 family transposase	-
K7X31_RS22600	44248..45069	+	822	WP_143189711.1	DUF932 domain-containing protein	-
K7X31_RS22605	45366..45956	-	591	WP_165850686.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeC / faeD / faeE / faeF / faeH / faeI / faeJ	1..89947	89947
	-	flank	IS/Tn	-	-	42759..43955	1196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T214720 WP_001372321.1 NZ_CP082358:41340-41465 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T214720 NZ_CP110198:364861-365136 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT214720 NZ_CP082358:c41261-41196 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin