Detailed information of TA system TA214718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4535609..4536211
• Replicon: chromosome
• Accession: NZ_CP082357
• Organism: Escherichia coli strain VCSW7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: K7X31_RS21445
• Protein ID: WP_000897305.1
• Coordinates: 4535900..4536211 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K7X31_RS21440
• Protein ID: WP_000356395.1
• Coordinates: 4535609..4535899 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X31_RS21410 (4531232)	4531232..4532134	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
K7X31_RS21415 (4532131)	4532131..4532766	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
K7X31_RS21420 (4532763)	4532763..4533692	+	930	WP_000027705.1	formate dehydrogenase accessory protein FdhE	-
K7X31_RS21425 (4533874)	4533874..4534116	-	243	WP_001086386.1	CopG family transcriptional regulator	-
K7X31_RS21430 (4534335)	4534335..4534553	-	219	WP_001295676.1	CopG family transcriptional regulator	-
K7X31_RS21435 (4534972)	4534972..4535250	-	279	WP_001306650.1	hypothetical protein	-
K7X31_RS21440 (4535609)	4535609..4535899	-	291	WP_000356395.1	NadS family protein	Antitoxin
K7X31_RS21445 (4535900)	4535900..4536211	-	312	WP_000897305.1	hypothetical protein	Toxin
K7X31_RS21450 (4536440)	4536440..4537348	+	909	WP_001306651.1	alpha/beta hydrolase	-
K7X31_RS22965 (4537517)	4537517..4538431	-	915	WP_262415893.1	transposase	-
K7X31_RS22970 (4538444)	4538444..4539331	-	888	Protein_4192	hypothetical protein	-
K7X31_RS21460 (4539747)	4539747..4540688	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
K7X31_RS21465 (4540733)	4540733..4541170	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T214718 WP_000897305.1 NZ_CP082357:c4536211-4535900 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T214718 NZ_CP110198:206805-207290 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT214718 WP_000356395.1 NZ_CP082357:c4535899-4535609 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT214718 NZ_CP110198:206530-206817 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7